Ultrastructure of dendritic spines: correlation between synaptic and spine morphologies.
Ultrastructure of dendritic spines: correlation between synaptic and spine morphologies.
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树突状棘的超微结构：突触和脊柱形态之间的相关性。
DOI：
10.3389/neuro.01.1.1.010.2007
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发表时间：
2007-11
影响因子：
4.3
通讯作者：
Yuste R
中科院分区：
文献类型：
--
作者：
Arellano JI;Benavides-Piccione R;Defelipe J;Yuste R
树突棘是皮层回路的关键元件，因为它们建立了大多数兴奋性突触。最近的研究报道了棘的形态和功能参数之间的相关性。具体而言，脊柱头部体积与突触后密度（PSD）的面积、突触后受体的数量和可释放的递质池相关，而脊柱颈部的长度与脊柱与其母树突的生物化学和电隔离的程度成比例。因此，棘的形态可以决定其突触强度和学习规则。为了更好地了解新皮质棘形态的自然变异性，我们使用了一个组合的金色调高尔基体和系列薄切片电子显微镜和进行三维重建的棘层2/3锥体细胞从小鼠视觉皮层。我们对144个完整的重建棘的结构和突触特征进行了表征，并根据它们的位置分析了它们的形态。对于所有的形态学参数分析，棘表现出连续的变异性，没有明确区分的亚型的棘或明确的依赖于他们的距离索马的形态。平均而言，脊柱头部体积与PSD面积强相关，与颈部直径弱相关，但与颈部长度无关。巨大的形态多样性表明突触强度和学习规则的变化同样大。
Dendritic spines are critical elements of cortical circuits, since they establish most excitatory synapses. Recent studies have reported correlations between morphological and functional parameters of spines. Specifically, the spine head volume is correlated with the area of the postsynaptic density (PSD), the number of postsynaptic receptors and the ready-releasable pool of transmitter, whereas the length of the spine neck is proportional to the degree of biochemical and electrical isolation of the spine from its parent dendrite. Therefore, the morphology of a spine could determine its synaptic strength and learning rules. To better understand the natural variability of neocortical spine morphologies, we used a combination of gold-toned Golgi impregnations and serial thin-section electron microscopy and performed three-dimensional reconstructions of spines from layer 2/3 pyramidal cells from mouse visual cortex. We characterized the structure and synaptic features of 144 completed reconstructed spines, and analyzed their morphologies according to their positions. For all morphological parameters analyzed, spines exhibited a continuum of variability, without clearly distinguishable subtypes of spines or clear dependence of their morphologies on their distance to the soma. On average, the spine head volume was correlated strongly with PSD area and weakly with neck diameter, but not with neck length. The large morphological diversity suggests an equally large variability of synaptic strength and learning rules.
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DOI：
10.1023/a:1024134312173
发表时间：
2002-03-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
Benavides-Piccione, R;Ballesteros-Yáñez, I;Yuste, R
通讯作者：
Yuste, R
DOI：
10.1007/bf01261506
发表时间：
1977-01-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
FIFKOVA, E;VANHARREVELD, A
通讯作者：
VANHARREVELD, A
影响因子：
3.3
作者：
Ballesteros-Yáñez, I;Benavides-Piccione, R;DeFelipe, J
通讯作者：
DeFelipe, J
影响因子：
56.9
作者：
Hensch, TK;Fagiolini, M;Kash, SF
通讯作者：
Kash, SF
DOI：
10.1101/sqb.1952.017.01.019
发表时间：
1952-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
CHANG, HT
通讯作者：
CHANG, HT