Cytokine storms are primarily responsible for the rapid death of ducklings infected with duck hepatitis A virus type 1.
Cytokine storms are primarily responsible for the rapid death of ducklings infected with duck hepatitis A virus type 1.
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细胞因子风暴是感染 1 型鸭甲型肝炎病毒的雏鸭快速死亡的主要原因
DOI：
10.1038/s41598-018-24729-w
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发表时间：
2018-04-26
影响因子：
4.6
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Xie J;Wang M;Cheng A;Zhao XX;Liu M;Zhu D;Chen S;Jia R;Yang Q;Wu Y;Zhang S;Liu Y;Yu Y;Zhang L;Sun K;Chen X
鸭甲型肝炎病毒1型（DHAV-1）是危害养鸭业最严重的病原之一。成年鸭感染DHAV-1之前被证明会导致肾脏中的短暂细胞因子风暴。为了了解DHAV-1感染如何影响宿主肝脏，我们用CH DHAV-1强毒株和减毒CH 60商业疫苗株进行了动物实验。通过肉眼观察和标准苏木精-伊红染色检测肝脏中的病理损伤，并通过定量PCR评估肝脏中的病毒拷贝数和细胞因子表达。CH株（108.4copies/mg）在肝脏中的病毒滴度高于CH 60株（104.9copies/mg），并在肝脏表面引起瘀斑。此外，来自接种CH菌株的雏鸭的肝脏被大量红细胞显著浸润，伴有严重的细胞因子风暴，但在接种CH 60菌株的雏鸭的肝脏中未观察到类似的体征。由此可见，CH株引起的细胞因子风暴可明显诱导雏鸭肝脏的出血性病变，这可能是雏鸭快速死亡的一个关键因素。
Duck hepatitis A virus type 1 (DHAV-1) is one of the most harmful pathogens in the duck industry. The infection of adult ducks with DHAV-1 was previously shown to result in transient cytokine storms in their kidneys. To understand how DHAV-1 infection impacts the host liver, we conducted animal experiments with the virulent CH DHAV-1 strain and the attenuated CH60 commercial vaccine strain. Visual observation and standard hematoxylin and eosin staining were performed to detect pathological damage in the liver, and viral copy numbers and cytokine expression in the liver were evaluated by quantitative PCR. The CH strain (108.4copies/mg) had higher viral titers than the CH60 strain (104.9copies/mg) in the liver and caused ecchymotic hemorrhaging on the liver surface. Additionally, livers from ducklings inoculated with the CH strain were significantly infiltrated by numerous red blood cells, accompanied by severe cytokine storms, but similar signs were not observed in the livers of ducklings inoculated with the CH60 strain. In conclusion, the severe cytokine storm caused by the CH strain apparently induces hemorrhagic lesions in the liver, which might be a key factor in the rapid death of ducklings.
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