TGase positively regulates photosynthesis via activation of Calvin cycle enzymes in tomato
TGase positively regulates photosynthesis via activation of Calvin cycle enzymes in tomato
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TGase 通过激活番茄卡尔文循环酶积极调节光合作用
DOI：
10.1038/s41438-019-0173-z
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发表时间：
2019-08
影响因子：
8.7
通讯作者：
Guo Shirong
中科院分区：
文献类型：
--
作者：
Zhong Min;Wang Yu;Hou Kun;Shu Sheng;Sun Jin;Guo Shirong
转谷氨酰胺酶（TGases）是广泛存在于植物中的一种交联酶，在植物光合作用和非生物/生物胁迫响应中起着重要作用，然而，关于TGase如何提高植物光合作用能力的遗传学证据以及TGase介导的光合作用机制尚不清楚。在这项研究中，我们通过比较野生型（WT）植物，由CRISPR/Cas9系统产生的TGase突变体和TGase-overexpressing（TGaseOE）植物，阐明了TGase在番茄光合作用调节中的功能。结果表明，TGase转录水平的提高导致净光合速率（Pn）的提高，而TGase突变体与WT相比，Pns和CO2同化作用显着抑制。TGaseOE植株的RuBisCO总活性不受TGase的影响，但其RuBisCO的初始和激活状态以及RuBisCO激活酶（RCA）和果糖-1，6-二磷酸酶（FBPase）活性均显著高于WT植株。除RuBisCO小亚基（RbcS）外，Benson-Calvin循环相关基因的转录水平与内源TGase活性呈正相关。此外，TGaseOE植物具有较高的蛋白质水平的RuBisCO大亚基（RbcL）和RCA比野生型植物，并表现出减少的氧化还原状态，通过增强脱氢抗坏血酸还原酶（达尔）和谷胱甘肽还原酶（GR），这是妥协的TGase缺陷的植物的活性。总的来说，TGase通过维持Benson-Calvin循环的激活状态和诱导番茄细胞氧化还原稳态的变化来正向调节光合作用。
Transglutaminases (TGases), which are widespread cross-linking enzymes in plants, play key roles in photosynthesis and abiotic/biotic stress responses; however, evidence concerning the genetics underlying how TGase improves the capability of photosynthesis and the mechanism of TGase-mediated photosynthesis are not clear in this crop species. In this study, we clarified the function of TGase in the regulation of photosynthesis in tomato by comparing wild-type (WT) plants, tgase mutants generated by the CRISPR/Cas9 system and TGase-overexpressing (TGaseOE) plants. Our results showed that increasing the transcript level of TGase resulted in an enhanced net photosynthetic rate (Pn), whereas the tgase mutants presented significantly inhibited Pns and CO 2 assimilation compared with the WT. Although the total RuBisCO activity was not affected by TGase, the initial and activation status of RuBisCO and the activity of RuBisCO activase (RCA) and fructose-1, 6-bisphosphatase (FBPase) in TGaseOE plants were significantly higher than that in WT plants. Except for RuBisCO small subunit (RbcS), the transcription levels of Benson–Calvin cycle-related genes were positively related to the endogenous TGase activity. Furthermore, TGaseOE plants had higher protein levels of RuBisCO large subunit (RbcL) and RCA than did WT plants and showed a reduced redox status by enhancing the activity of dehydroascorbate reductase (DHAR) and glutathione reductase (GR), which was compromised in TGase-deficient plants. Overall, TGase positively regulated photosynthesis by maintaining the activation states of the Benson–Calvin cycle and inducing changes in cellular redox homeostasis in tomato.
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影响因子：
6.9
作者：
Cheng F;Zhou YH;Xia XJ;Shi K;Zhou J;Yu JQ
通讯作者：
Yu JQ
腐胺在盐胁迫下调节类囊体膜蛋白质和脂肪酸中的作用
影响因子：
4.6
作者：
Shu S;Yuan Y;Chen J;Sun J;Zhang W;Tang Y;Zhong M;Guo S
通讯作者：
Guo S
影响因子：
4.9
作者：
Y. Nakano;K. Asada
通讯作者：
Y. Nakano;K. Asada
影响因子：
7.4
作者：
Della Mea, M;Caparrós-Ruiz, D;Rigau, J
通讯作者：
Rigau, J
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
K. Livak;Thomas D. Schmittgen
通讯作者：
K. Livak;Thomas D. Schmittgen