Efficient preparation of rare Sagittatoside A from epimedin A, by recyclable aqueous organic two-phase enzymatic hydrolysis
Efficient preparation of rare Sagittatoside A from epimedin A, by recyclable aqueous organic two-phase enzymatic hydrolysis
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利用可回收水有机两相酶解法从淫羊藿A高效制备稀有矢车菊苷A
DOI：
10.1080/14786419.2018.1519820
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发表时间：
2018-11
影响因子：
2.2
通讯作者：
Xiaobin Jia
中科院分区：
文献类型：
--
作者：
Yuping Shen;Yi Lu;Jing Gao;Yeting Zhu;Man Wang;Shunli Jing;Lili Xu;Huan Yang;Xiaobin Jia
摘要慈姑苷A是一种罕见的次生黄酮醇苷，其体内生物活性远高于朝藿定A。然而，目前的制备方法效率低，且有副产物产生。本研究旨在建立一种基于可循环、集成的水相有机两相酶解反应的新型催化体系，以方便有效地从朝藿定A制备慈姑苷A。以乙酸丙酯和含β-葡聚糖酶/朝藿定A的HAc-NaAc缓冲液（pH4.5）为体系，60 ℃水解1h。因此，朝藿定A被完全水解为慈姑苷A，并且95.02%的产物被转移到有机相中。此外，在七个水解循环后，其初始活性保留了90%。此外，该方法比传统的酶解方法简单。总的来说，新提出的策略是一个有效的和有前途的方法，在工业应用中制备慈姑苷A。
Abstract The rare secondary flavonol glycoside Sagittatoside A has much better in vivo bioactivities than epimedin A in Epimedii Folium. However, its current preparation methods are of low efficiency, with byproducts generated. The aim of this study was to establish a novel catalysis system for effective and convenient preparation of Sagittatoside A from epimedin A based on recyclable and integrated aqueous organic two-phase enzymatic hydrolysis. The system was consisted of propyl acetate and HAc-NaAc buffer (pH4.5) containing β-dextranase/epimedin A, and the hydrolysis was performed at 60 °C for 1 h. Consequently, epimedin A was completely hydrolyzed to sagittatoside A, and 95.02% of the product was transferred into the organic phase. Moreover, 90% of its initial activity was retained after seven cycles of hydrolysis. Additionally, the procedure was simpler than conventional enzymatic hydrolysis. Collectively, the newly proposed strategy is an efficient and promising approach for the preparation of sagittatoside A in industrial application.
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