Effective Components of Panax quinquefolius and Corydalis tuber Protect Myocardium through Attenuating Oxidative Stress and Endoplasmic Reticulum Stress.
Effective Components of Panax quinquefolius and Corydalis tuber Protect Myocardium through Attenuating Oxidative Stress and Endoplasmic Reticulum Stress.
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DOI：
10.1155/2013/482318
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Yin H
中科院分区：
文献类型：
--
作者：
Xue M;Liu M;Zhu X;Yang L;Miao Y;Shi D;Yin H
氧化应激和内质网应激（ERS）均参与了肿瘤的发生和神经系统疾病的发生，但其在急性心肌梗死（AMI）发生发展中的作用机制尚未见报道。本研究观察了氧化应激和ERS在大鼠急性心肌梗死模型中的作用，并评价了美托洛尔和西洋参有效成分（EPC）在治疗中的作用。采用结扎大鼠冠状动脉左前降支的方法建立急性心肌梗死模型。给药2周后，采用酶联免疫吸附法（ELISA）检测各组大鼠血清丙二醛（MDA）、超氧化物歧化酶（SOD）和8-异前列腺素F2α（8-iso-PGF 2 α）水平。采用实时荧光定量PCR和Western blotting检测ERS-glucose-regulated protein-78（GRP 78）和CCAAT/enhancer-binding protein homologous protein（CHOP）的mRNA和蛋白表达。结果证实，美托洛尔和中、高剂量EPC均能降低血清8-iso-PGF 2 α水平，下调心肌GRP 78和CHOP的mRNA和蛋白表达，而EPC还能升高血清SOD水平。这些结果表明，美托洛尔和EPC通过减轻心肌梗死诱导的氧化应激和ERS来保护心肌，强调ERS途径是AMI的潜在治疗靶点。
Both oxidative stress and endoplasmic reticulum stress (ERS) have been implicated in carcinogenesis and neurological diseases, while there are few reports about the mechanisms of them in the progression of acute myocardial infarction (AMI). This study examined oxidative stress and ERS in a rat model of AMI and evaluated their role in therapy by metoprolol and effective components of Panax quinquefolius and Corydalis tuber (EPC). In the present study a rat model of AMI was established by ligation of the left anterior descending coronary artery. After oral administration of metoprolol or low-to-high doses of EPC for 2 weeks, serum malondialdehyde (MDA), superoxide dismutase (SOD), and 8-iso-prostaglandin F2α (8-iso-PGF2α) were detected using enzyme-linked immunosorbent assay (ELISA). Quantitative real-time PCR and Western blotting were used to examine mRNA and protein expressions of the hallmarks of ERS-glucose-regulated protein-78 (GRP78) and CCAAT/enhancer-binding protein homologous protein (CHOP). We confirmed that both metoprolol and moderate-to-high dose of EPC decreased 8-iso-PGF2α serum level and downregulated the mRNA and protein expressions of GRP78 and CHOP in myocardium, while EPC also increased SOD serum level. These results indicated that metoprolol and EPC protect the myocardium by attenuating oxidative stress and ERS induced by myocardial infarction, highlighting the ERS pathways as potential therapeutic targets for AMI.
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影响因子：
10.8
作者：
George, Isaac;Sabbah, Hani N.;Wang, Jie
通讯作者：
Wang, Jie
影响因子：
2.7
作者：
Garelnabi, Mahdi;Gupta, Vinod;Bhattacharjee, Jayashree
通讯作者：
Bhattacharjee, Jayashree
DOI：
10.1007/978-1-59745-364-6_14
发表时间：
2010-01-01
期刊：
CYCLOOXYGENASES: METHODS AND PROTOCOLS
影响因子：
--
作者：
Tacconelli, Stefania;Capone, Marta L.;Patrignani, Paola
通讯作者：
Patrignani, Paola
影响因子：
7.4
作者：
van Deel, Eiza D.;Lu, Zhongbing;Chen, Yingjie
通讯作者：
Chen, Yingjie
影响因子：
3.1
作者：
Wang, Chen;Li, Yu-Zhen;Liu, Xiu-Hua
通讯作者：
Liu, Xiu-Hua