Natural variation in ZmNAC087 contributes to total root length regulation in maize seedlings under salt stress.
Natural variation in ZmNAC087 contributes to total root length regulation in maize seedlings under salt stress.
复制标题
ZmNAC087的自然变异有助于盐胁迫下玉米幼苗总根长的调节。
DOI：
10.1186/s12870-023-04393-7
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发表时间：
2023-08-14
影响因子：
5.3
通讯作者：
Li, Pengcheng
中科院分区：
文献类型：
--
作者：
Zhang, Xiaomin;Wang, Houmiao;Yang, Mengling;Liu, Runxiao;Zhang, Xin;Jia, Zhongtao;Li, Pengcheng
土壤盐分对作物生长和生产力构成重大挑战，特别是影响对水分和养分吸收至关重要的根系。为了确定胁迫环境下影响根系伸长的遗传因素，我们进行了一项全基因组关联研究（GWAS），研究了盐胁迫和正常条件下玉米幼苗总根长（TRL）的自然变异。本研究共鉴定出与38个候选基因相关的69个遗传变异，其中zmac087中一个特异性单核苷酸多态性（SNP）与盐胁迫下TRL显著相关。瞬时表达和反活化实验表明，zmac087编码一个具有反活化活性的核定位蛋白。进一步的候选基因关联分析表明，ZmNAC087启动子的非编码变异导致了ZmNAC087在玉米自交系间的差异表达，可能影响盐调控TRL的变异。此外，通过核苷酸多样性分析、中性测试和聚结模拟，我们证明了zmac087在玉米驯化和改良过程中经历了选择。这些发现突出了zmac087天然变异，特别是有利等位基因在玉米耐盐性中的重要意义，为玉米耐盐种质资源的开发提供了理论依据和宝贵的遗传资源。在线版本包含补充材料，下载地址：10.1186/s12870-023-04393-7。
Soil salinity poses a significant challenge to crop growth and productivity, particularly affecting the root system, which is vital for water and nutrient uptake. To identify genetic factors that influence root elongation in stressful environments, we conducted a genome-wide association study (GWAS) to investigate the natural variation associated with total root length (TRL) under salt stress and normal conditions in maize seedlings. Our study identified 69 genetic variants associated with 38 candidate genes, among which a specific single nucleotide polymorphism (SNP) in ZmNAC087 was significantly associated with TRL under salt stress. Transient expression and transactivation assays revealed that ZmNAC087 encodes a nuclear-localized protein with transactivation activity. Further candidate gene association analysis showed that non-coding variations in ZmNAC087 promoter contribute to differential ZmNAC087 expression among maize inbred lines, potentially influencing the variation in salt-regulated TRL. In addition, through nucleotide diversity analysis, neutrality tests, and coalescent simulation, we demonstrated that ZmNAC087 underwent selection during maize domestication and improvement. These findings highlight the significance of natural variation in ZmNAC087, particularly the favorable allele, in maize salt tolerance, providing theoretical basis and valuable genetic resources for the development of salt-tolerant maize germplasm. The online version contains supplementary material available at 10.1186/s12870-023-04393-7.
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DOI：
10.3390/plants12040690
发表时间：
2023-02-04
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.3
作者：
Cramer GR;Urano K;Delrot S;Pezzotti M;Shinozaki K
通讯作者：
Shinozaki K
影响因子：
--
作者：
Habib SH;Kausar H;Saud HM
通讯作者：
Saud HM
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
影响因子：
7.2
作者：
Hao, Yu-Jun;Wei, Wei;Chen, Shou-Yi
通讯作者：
Chen, Shou-Yi