Influenza H5 hemagglutinin DNA primes the antibody response elicited by the live attenuated influenza A/Vietnam/1203/2004 vaccine in ferrets.
Influenza H5 hemagglutinin DNA primes the antibody response elicited by the live attenuated influenza A/Vietnam/1203/2004 vaccine in ferrets.
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DOI：
10.1371/journal.pone.0021942
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Lu S
中科院分区：
文献类型：
--
作者：
Suguitan AL Jr;Cheng X;Wang W;Wang S;Jin H;Lu S
引发免疫在保护个体或群体免受人类新流感病毒的侵害方面起着关键作用。为了确定最有前途的疫苗引发策略，我们已经评估了不同的引发-加强方案使用灭活，DNA和活减毒疫苗在雪貂。甲型流感/Vietnam/1203/2004（H5 N1）减毒活候选疫苗（LAIV，VN 04 ca）有效地引发了雪貂，而灭活H5 N1疫苗无法引发血清阴性雪貂的免疫反应，除非使用佐剂。然而，单独的H5 HA DNA疫苗在引发对VN 04 ca病毒的免疫应答方面与佐剂灭活的VN 04疫苗一样成功。用H5 HA DNA致敏，然后鼻内接种VN 04 ca病毒的雪貂的血清抗体滴度与两剂VN 04 ca病毒诱导的血清抗体滴度相当。LAIV-LAIV和DNA-LAIV疫苗方案均能诱导抗体应答，交叉中和抗原性不同的H5 N1病毒分离株，包括A/HongKong/213/2003（HK 03），并预防HK 03疫苗病毒的鼻感染。因此，H5 HA DNA疫苗接种可能为大流行防范提供另一种选择。
Priming immunization plays a key role in protecting individuals or populations to influenza viruses that are novel to humans. To identify the most promising vaccine priming strategy, we have evaluated different prime-boost regimens using inactivated, DNA and live attenuated vaccines in ferrets. Live attenuated influenza A/Vietnam/1203/2004 (H5N1) candidate vaccine (LAIV, VN04 ca) primed ferrets efficiently while inactivated H5N1 vaccine could not prime the immune response in seronegative ferrets unless an adjuvant was used. However, the H5 HA DNA vaccine alone was as successful as an adjuvanted inactivated VN04 vaccine in priming the immune response to VN04 ca virus. The serum antibody titers of ferrets primed with H5 HA DNA followed by intranasal vaccination of VN04 ca virus were comparable to that induced by two doses of VN04 ca virus. Both LAIV-LAIV and DNA-LAIV vaccine regimens could induce antibody responses that cross-neutralized antigenically distinct H5N1 virus isolates including A/HongKong/213/2003 (HK03) and prevented nasal infection of HK03 vaccine virus. Thus, H5 HA DNA vaccination may offer an alternative option for pandemic preparedness.
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