Skeletal muscle explants: ex-vivo models to study cellular behavior in a complex tissue environment.

Skeletal muscle explants: ex-vivo models to study cellular behavior in a complex tissue environment.
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DOI:
10.1080/03008207.2019.1662409
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发表时间:
2020-05
影响因子:
2.9
通讯作者:
Meyer GA
Meyer GA
中科院分区:
医学3区
文献类型:
--
作者:
Smith LR;Meyer GA

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骨骼肌组织外植体的培养和研究已有近 100 年的历史。这些培养物在适合精确操作和测量的环境中保留了复杂的组织结构,导致了调节收缩性、代谢和再生的外在和内在机制的开创性发现。本综述讨论了肌肉外植体的两种主要模型:分离的肌纤维和完整的肌肉。它回顾了每种技术的独特挑战和能力,重点关注当前和未来有影响力的新颖应用。
Skeletal muscle tissue explants have been cultured and studied for nearly 100 years. These cultures, which retain complex tissue structure in an environment suited to precision manipulation and measurement, have led to seminal discoveries of the extrinsic and intrinsic mechanisms regulating contractility, metabolism and regeneration. This review discusses the two primary models of muscle explant: isolated myofiber and intact muscle. It reviews the unique challenges and capabilities of each, with a focus on impactful current and future novel applications.
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