Significant improvement in cloning efficiency of an inbred miniature pig by histone deacetylase inhibitor treatment after somatic cell nuclear transfer.
Significant improvement in cloning efficiency of an inbred miniature pig by histone deacetylase inhibitor treatment after somatic cell nuclear transfer.
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DOI：
10.1095/biolreprod.109.077016
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发表时间：
2009-09
影响因子：
3.6
通讯作者：
Prather RS
中科院分区：
文献类型：
--
作者：
Zhao J;Ross JW;Hao Y;Spate LD;Walters EM;Samuel MS;Rieke A;Murphy CN;Prather RS
The NIH miniature pig was developed specifically for xenotransplantation and has been extensively used as a large animal model in many other biomedical experiments. However the cloning efficiency of this pig is very low (less than 0.2%) and this has been an obstacle to the promising application of these inbred swine genetics for biomedical research. It has been demonstrated that increased histone acetylation in somatic cell nuclear transfer (SCNT) embryos, by applying histone deacetylase inhibitors (HDACi) such as trichostatin A (TSA), significantly enhances the developmental competence in several species. However some researchers also reported that TSA treatment had various detrimental effects on the in vitro and in vivo development of the SCNT embryos. Here we report that treatment with 500 nM Scriptaid, a novel HDACi, significantly enhanced the development SCNT embryos to the blastocyst stage when NIH inbred fetal fibroblast cells (FFCs) (21% vs. 9%, P < 0.05) were used as donors compared to the untreated group. Scriptaid treatment resulted in 8 pregnancies from 10 embryo transfers (ET) and 14 healthy NIH miniature pigs from 8 litters while no viable piglets (only 3 mummies) were obtained from 9 ETs in the untreated group. Thus Scriptaid dramatically increased the cloning efficiency when using inbred genetics from zero to 1.3%. In contrast, Scriptaid treatment decreased the blastocyst rate in IVF embryos (from 37% to 26%, P < 0.05). In conclusion, the extreme low cloning efficiency in the NIH miniature pig may be caused by its inbred genetic background and can be improved by alteration of genomic histone acetylation patterns.
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影响因子：
--
作者：
Kishigami, Satoshi;Ohta, Hiroshi;Wakayama, Teruhiko
通讯作者：
Wakayama, Teruhiko
DOI：
10.1530/jrf.0.0820595
发表时间：
1988-03-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
CONLEY, AJ;JUNG, YC;FORD, SP
通讯作者：
FORD, SP
影响因子：
1.8
作者：
Kurome, Mayuko;Ishikawa, Takayuki;Nagashima, Hiroshi
通讯作者：
Nagashima, Hiroshi
影响因子：
6.2
作者：
Mezrich, JD;Haller, GW;Sachs, DH
通讯作者：
Sachs, DH
影响因子：
3.6
作者：
Inoue, K;Ogonuki, N;Ogura, A
通讯作者：
Ogura, A