In vitro proliferation of primitive hemopoietic stem cells supported by stromal cells: evidence for the presence of a mechanism(s) other than that involving c-kit receptor and its ligand.
In vitro proliferation of primitive hemopoietic stem cells supported by stromal cells: evidence for the presence of a mechanism(s) other than that involving c-kit receptor and its ligand.
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DOI：
10.1084/jem.176.2.351
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发表时间：
1992-08-01
影响因子：
15.3
通讯作者：
NISHIKAWA, S
中科院分区：
文献类型：
--
作者：
KODAMA, H;NOSE, M;YAMAGUCHI, Y;TSUNODA, J;SUDA, T;NISHIKAWA, S;NISHIKAWA, S
前脂肪细胞系PA 6可以支持长期造血。在骨髓细胞和PA 6细胞共培养6周后，能够维持造血的造血干细胞的频率为1/5.3 × 10（4）个骨髓细胞。在以2.5 × 104个细胞/皿接种骨髓细胞的培养皿组中，19个培养皿中的3个（16%）含有能够重建WBB 6 F1-W/Wv小鼠红细胞生成的干细胞，表明PA 6细胞可以支持原始造血干细胞的增殖。当用拮抗性抗c-kit单克隆抗体ACK 2处理共培养物时，只有少量的第12天脾集落形成单位存活;并且造血严重减少。然而，当用无抗体培养基继续共培养时，造血显著恢复。为了检查ACK 2抗性干细胞的增殖特性，我们通过修改我们的共培养系统开发了集落测定系统。对单个集落的发育及其消失的连续观察表明，具有较高增殖能力的干细胞优先在ACK 2处理后存活。此外，不能支持长期造血的PA 6克隆的亚克隆的细胞表达c-kit配体的mRNA。这些结果表明，在原始造血干细胞的存活和增殖中，除了c-kit受体及其配体的作用机制外，还有其他机制起着重要作用。
The preadipose cell line, PA6, can support long-term hemopoiesis. Frequency of the hemopoietic stem cells capable of sustaining hemopoiesis in cocultures of bone marrow cells and PA6 cells for 6 wk was 1/5.3 x 10(4) bone marrow cells. In the group of dishes into which bone marrow cells had been inoculated at 2.5 x 10(4) cells/dish, 3 of 19 dishes (16%) contained stem cells capable of reconstituting erythropoiesis of WBB6F1-W/Wv mice, indicating that PA6 cells can support the proliferation of primitive hemopoietic stem cells. When the cocultures were treated with an antagonistic anti-c-kit monoclonal antibody, ACK2, only a small number of day 12 spleen colony-forming units survived; and hemopoiesis was severely reduced. However, when the cocultures were continued with antibody-free medium, hemopoiesis dramatically recovered. To examine the proliferative properties of the ACK2-resistant stem cells, we developed a colony assay system by modifying our coculture system. Sequential observations of the development of individual colonies and their disappearance demonstrated that the stem cells having higher proliferative capacity preferentially survive the ACK2 treatment. Furthermore, cells of subclones of the PA6 clone that were incapable of supporting long-term hemopoiesis expressed mRNA for the c-kit ligand. These results suggest that a mechanism(s) other than that involving c-kit receptor and its ligand plays an important role in the survival and proliferation of primitive hemopoietic stem cells.
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影响因子：
64.5
作者：
HUANG, E;NOCKA, K;BESMER, P
通讯作者：
BESMER, P
影响因子：
64.8
作者：
DEXTER, TM;SPOONCER, E
通讯作者：
SPOONCER, E
影响因子：
11.4
作者：
NISHIKAWA, S;KUSAKABE, M;NISHIKAWA, SI
通讯作者：
NISHIKAWA, SI
DOI：
10.1084/jem.174.1.63
发表时间：
1991-07-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ogawa M;Matsuzaki Y;Nishikawa S;Hayashi S;Kunisada T;Sudo T;Kina T;Nakauchi H;Nishikawa S
通讯作者：
Nishikawa S
影响因子：
8.8
作者：
PORTER, E H;BERRY, R J
通讯作者：
BERRY, R J