Effect of neutrophil depletion on gelatinase expression, edema formation and hemorrhagic transformation after focal ischemic stroke.
Effect of neutrophil depletion on gelatinase expression, edema formation and hemorrhagic transformation after focal ischemic stroke.
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DOI：
10.1186/1471-2202-6-49
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发表时间：
2005-08-03
期刊：
影响因子：
2.4
通讯作者：
Fagan SC
中科院分区：
文献类型：
--
作者：
Harris AK;Ergul A;Kozak A;Machado LS;Johnson MH;Fagan SC
虽然局灶性脑缺血再灌注损伤后明胶酶(MMP2和MMP9)活性升高，但中性粒细胞对MMP活性和出血性转化的相对贡献尚不清楚。抗中性粒细胞治疗导致接受治疗的动物中性粒细胞的成功耗竭。对照组和PMN耗竭组在脑梗塞体积和出血方面均无差异。虽然缺血后明胶酶(MMP2和MMP9)的表达和活性显著增加，并形成水肿，但中性粒细胞的去除并未引起任何变化。本研究的主要发现是，在缺乏循环中性粒细胞的情况下，局灶性脑缺血后基质金属蛋白酶-2和基质金属蛋白酶-9的表达和活性仍然上调。此外，去除中性粒细胞对包括水肿、出血和梗塞范围在内的缺血性脑损伤的指标没有影响。这些发现表明，至少在急性情况下，中性粒细胞并不是与中风后急性神经血管损伤相关的明胶酶活性的重要贡献者。
While gelatinase (MMP-2 and -9) activity is increased after focal ischemia/reperfusion injury in the brain, the relative contribution of neutrophils to the MMP activity and to the development of hemorrhagic transformation remains unknown. Anti-PMN treatment caused successful depletion of neutrophils in treated animals. There was no difference in either infarct volume or hemorrhage between control and PMN depleted animals. While there were significant increases in gelatinase (MMP-2 and MMP-9) expression and activity and edema formation associated with ischemia, neutrophil depletion failed to cause any change. The main finding of this study is that, in the absence of circulating neutrophils, MMP-2 and MMP-9 expression and activity are still up-regulated following focal cerebral ischemia. Additionally, neutrophil depletion had no influence on indicators of ischemic brain damage including edema, hemorrhage, and infarct size. These findings indicate that, at least acutely, neutrophils are not a significant contributor of gelatinase activity associated with acute neurovascular damage after stroke.
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影响因子：
2.1
作者：
Smeglin, Anthony;Frishman, William H
通讯作者：
Frishman, William H
DOI：
10.1097/00004647-199906000-00005
发表时间：
1999-06-01
影响因子：
6.3
作者：
Heo, JH;Lucero, J;del Zoppo, GJ
通讯作者：
del Zoppo, GJ
影响因子：
1.9
作者：
Fagan, SC;Nagaraja, TN;Knight, RA
通讯作者：
Knight, RA
影响因子：
8.3
作者：
BEDERSON, JB;PITTS, LH;BARTKOWSKI, H
通讯作者：
BARTKOWSKI, H
影响因子：
8.3
作者：
HAMANN, GF;OKADA, Y;DELZOPPO, GJ
通讯作者：
DELZOPPO, GJ