X-ray micro-computed tomography in willow reveals tissue patterning of reaction wood and delay in programmed cell death.
X-ray micro-computed tomography in willow reveals tissue patterning of reaction wood and delay in programmed cell death.
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柳树中的X射线微型计算层析成像揭示了反应木材的组织模式和程序性细胞死亡的延迟。
DOI：
10.1186/s12870-015-0438-0
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发表时间：
2015-03-11
影响因子：
5.3
通讯作者：
Murphy RJ
中科院分区：
文献类型：
--
作者：
Brereton NJ;Ahmed F;Sykes D;Ray MJ;Shield I;Karp A;Murphy RJ
反应木材（RW）响应的变化已被证明是驱动来自生物能源作物杨柳的木质纤维素糖产量差异的主要成分。然而，糖产量差异背后的表型原因，除了它们共同的激发子之外，仍然不清楚。在这里，我们使用X射线显微计算机断层扫描（μCT），以调查RW相关的变化在次生木质部组织图案的三维（3D）。主要的建筑改造成功地量化了3D，并归因于RW诱导。虽然在张力木材组织（TW）的血管的频率减少，总的血管体积显着增加。有趣的是，还清楚地观察到与TW相关的程序性细胞死亡（PCD）延迟，并易于通过μCT进行定量。令人惊讶的程度，导管的体积增加说明了大量的木质部组织改建参与反应木材的形成。重塑表明生物量的广泛改变所必需的结构和水力结构之间的重要生理妥协，并有助于证明改善我们对细胞和组织结构的看法的力量。对木质部组织发育的精确观察和对PCD延迟程度的量化为这种生物能源作物性状提供了有价值的和令人兴奋的见解。本文的在线版本（doi：10.1186/s12870-015-0438-0）包含补充材料，可供授权用户使用。
Variation in the reaction wood (RW) response has been shown to be a principle component driving differences in lignocellulosic sugar yield from the bioenergy crop willow. The phenotypic cause(s) behind these differences in sugar yield, beyond their common elicitor, however, remain unclear. Here we use X-ray micro-computed tomography (μCT) to investigate RW-associated alterations in secondary xylem tissue patterning in three dimensions (3D). Major architectural alterations were successfully quantified in 3D and attributed to RW induction. Whilst the frequency of vessels was reduced in tension wood tissue (TW), the total vessel volume was significantly increased. Interestingly, a delay in programmed-cell-death (PCD) associated with TW was also clearly observed and readily quantified by μCT. The surprising degree to which the volume of vessels was increased illustrates the substantial xylem tissue remodelling involved in reaction wood formation. The remodelling suggests an important physiological compromise between structural and hydraulic architecture necessary for extensive alteration of biomass and helps to demonstrate the power of improving our perspective of cell and tissue architecture. The precise observation of xylem tissue development and quantification of the extent of delay in PCD provides a valuable and exciting insight into this bioenergy crop trait. The online version of this article (doi:10.1186/s12870-015-0438-0) contains supplementary material, which is available to authorized users.
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影响因子：
1.9
作者：
Donaldson, Lloyd
通讯作者：
Donaldson, Lloyd
影响因子：
6.3
作者：
Brereton NJ;Pitre FE;Ray MJ;Karp A;Murphy RJ
通讯作者：
Murphy RJ
影响因子：
7.2
作者：
Courtois-Moreau, Charleen L.;Pesquet, Edouard;Tuominen, Hannele
通讯作者：
Tuominen, Hannele
影响因子：
3
作者：
Mayo, S. C.;Chen, F.;Evans, R.
通讯作者：
Evans, R.
影响因子：
4.3
作者：
PRODHAN, AKMA;FUNADA, R;FUKAZAWA, K
通讯作者：
FUKAZAWA, K