Interactions between horizontally acquired genes create a fitness cost in Pseudomonas aeruginosa.
Interactions between horizontally acquired genes create a fitness cost in Pseudomonas aeruginosa.
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DOI：
10.1038/ncomms7845
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发表时间：
2015-04-21
影响因子：
16.6
通讯作者：
MacLean, R. Craig
中科院分区：
文献类型：
--
作者：
Millan, Alvaro San;Toll-Riera, Macarena;Qi, Qin;MacLean, R. Craig
水平基因转移（HGT）在细菌进化中起着关键作用，特别是在抗生素耐药性方面。与移动的遗传因素（MGE）相关的健身成本被认为是限制HGT的因素，但我们对这些成本的理解仍然是零碎的，因此很难预测HGT事件的成功。在这里，我们使用铜绿假单胞菌和一个昂贵的质粒（pNUK 73）之间的相互作用，以调查HGT的成本的分子基础。使用RNA-Seq，我们表明，收购pNUK 73的结果在染色体基因的转录谱的深刻改变。染色体整合的MGE上编码的两个基因的突变通过降低pNUK 73复制基因的表达来恢复这些适应性成本和转录变化。我们的研究表明，MGE之间的相互作用可以通过改变基因表达来损害细菌的适应性，我们认为，移动的元素之间的冲突对HGT的进化施加了普遍的约束。 水平基因转移对细菌进化很重要，但其适应性代价的分子基础仍不清楚。在这里，作者表明，铜绿假单胞菌中质粒产生的适应性成本被最近获得的编码移动的遗传元件的基因突变所减轻。
Horizontal gene transfer (HGT) plays a key role in bacterial evolution, especially with respect to antibiotic resistance. Fitness costs associated with mobile genetic elements (MGEs) are thought to constrain HGT, but our understanding of these costs remains fragmentary, making it difficult to predict the success of HGT events. Here we use the interaction between P. aeruginosa and a costly plasmid (pNUK73) to investigate the molecular basis of the cost of HGT. Using RNA-Seq, we show that the acquisition of pNUK73 results in a profound alteration of the transcriptional profile of chromosomal genes. Mutations that inactivate two genes encoded on chromosomally integrated MGEs recover these fitness costs and transcriptional changes by decreasing the expression of the pNUK73 replication gene. Our study demonstrates that interactions between MGEs can compromise bacterial fitness via altered gene expression, and we argue that conflicts between mobile elements impose a general constraint on evolution by HGT. Horizontal gene transfer is important for bacterial evolution but the molecular basis of its fitness costs remain unclear. Here the authors show that fitness costs produced by a plasmid in P. aeruginosa are alleviated by mutations in recently acquired genes encoded in mobile genetic elements.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
DOI：
10.1073/pnas.0801269105
发表时间：
2008-05-13
影响因子：
11.1
作者：
Gaillard, Muriel;Pernet, Nataskha;van der Meer, Jan Roelof
通讯作者：
van der Meer, Jan Roelof
影响因子：
3.2
作者：
Klockgether, Jens;Munder, Antje;Tuemmler, Burkhard
通讯作者：
Tuemmler, Burkhard
影响因子：
10.7
作者：
Cohen, Ofir;Gophna, Uri;Pupko, Tal
通讯作者：
Pupko, Tal
影响因子：
14.9
作者：
Balasubramanian D;Kumari H;Jaric M;Fernandez M;Turner KH;Dove SL;Narasimhan G;Lory S;Mathee K
通讯作者：
Mathee K