Identification of 3-Sulfinopropionyl Coenzyme A (CoA) Desulfinases within the Acyl-CoA Dehydrogenase Superfamily
Identification of 3-Sulfinopropionyl Coenzyme A (CoA) Desulfinases within the Acyl-CoA Dehydrogenase Superfamily
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酰基辅酶 A 脱氢酶超家族中 3-磺基丙酰辅酶 A (CoA) 脱硫酶的鉴定
DOI：
10.1128/jb.01265-13
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发表时间：
2014
影响因子：
3.2
通讯作者：
Steinbüchel
中科院分区：
文献类型：
--
作者：
Schürmann;Demming;Krewing;Wübbeler;Steinbüchel
在先前的研究中，阐明了Advenella mimigardefordensis菌株DPN7T（AcdDPN7）中3-亚磺基丙酰辅酶A（3SP-CoA）脱硫酶酰基辅酶A脱氢酶（Acd）在降解3,3'-二硫代二丙酸（DTDP）过程中的重要作用。 DTDP 是一种用于生物合成聚硫酯 (PTE) 的含硫前体底物。 AcdDPN7 显示出与酰基辅酶A脱氢酶高度相似的氨基酸序列，但无法催化脱氢反应。因此，本研究调查了 3SP-CoA 脱硫酶活性在酰基辅酶 A 脱氢酶超家族中是否是罕见或广泛的特性。因此，来自克什米尔 Advenella kashmirensis WT001、蜡样芽孢杆菌 DSM31、Cupriavidus necator N-1、大肠杆菌 BL21、恶臭假单胞菌 KT2440、异食伯克霍尔德氏菌 LB400、Ralstonia eutropica H16、Variovorax paradoxus B4 的酰基辅酶A脱氢酶超家族的蛋白质， Variovorax paradoxus S110 和 Variovorax paradoxus TBEA6 在大肠杆菌菌株中表达。如尺寸排阻色谱法所示，所有纯化的酰基辅酶A脱氢酶均表现为同源四聚体。 AcdS110、AcdB4、AcdH16 和 AcdKT2440 能够使异丁酰辅酶 A 脱氢。 AcdKT2440另外使丁酰辅酶A和戊酰辅酶A脱氢，而AcdDSM31仅使丁酰辅酶A和戊酰辅酶A脱氢。对于任何所研究的酰基辅酶A脱氢酶，均未观察到丙酰辅酶A、异戊酰辅酶A、琥珀酰辅酶A和戊二酰辅酶A的脱氢反应。只有 AcdTBEA6、AcdN-1 和 AcdLB400 使 3SP-CoA 脱硫，因此被鉴定为酰基辅酶 A 脱氢酶家族中的 3SP-CoA 脱硫酶，尽管这三种 Acd 都不能使任何测试的酰基辅酶 A 硫酯脱氢。没有鉴定出适合 AcdBL21 和 AcdWT001 的底物。分光光度测定提供了活性底物的表观 KmandVmax 值，并表明系统发育分析可用于预测未表征的酰基辅酶 A 脱氢酶的底物范围。此外，C. necator N-1被发现利用3SP作为碳和能量的唯一来源。
In a previous study, the essential role of 3-sulfinopropionyl coenzyme A (3SP-CoA) desulfinase acyl-CoA dehydrogenase (Acd) in Advenella mimigardefordensis strain DPN7T(AcdDPN7) during degradation of 3,3′-dithiodipropionic acid (DTDP) was elucidated. DTDP is a sulfur-containing precursor substrate for biosynthesis of polythioesters (PTEs). AcdDPN7showed high amino acid sequence similarity to acyl-CoA dehydrogenases but was unable to catalyze a dehydrogenation reaction. Hence, it was investigated in the present study whether 3SP-CoA desulfinase activity is an uncommon or a widespread property within the acyl-CoA dehydrogenase superfamily. Therefore, proteins of the acyl-CoA dehydrogenase superfamily from Advenella kashmirensis WT001, Bacillus cereus DSM31, Cupriavidus necator N-1, Escherichia coli BL21, Pseudomonas putida KT2440, Burkholderia xenovorans LB400, Ralstonia eutropha H16, Variovorax paradoxus B4, Variovorax paradoxus S110, and Variovorax paradoxus TBEA6 were expressed in E. coli strains. All purified acyl-CoA dehydrogenases appeared as homotetramers, as revealed by size exclusion chromatography. AcdS110, AcdB4, AcdH16, and AcdKT2440were able to dehydrogenate isobutyryl-CoA. AcdKT2440additionally dehydrogenated butyryl-CoA and valeryl-CoA, whereas AcdDSM31dehydrogenated only butyryl-CoA and valeryl-CoA. No dehydrogenation reactions were observed with propionyl-CoA, isovaleryl-CoA, succinyl-CoA, and glutaryl-CoA for any of the investigated acyl-CoA dehydrogenases. Only AcdTBEA6, AcdN-1, and AcdLB400desulfinated 3SP-CoA and were thus identified as 3SP-CoA desulfinases within the acyl-CoA dehydrogenase family, although none of these three Acds dehydrogenated any of the tested acyl-CoA thioesters. No appropriate substrates were identified for AcdBL21and AcdWT001. Spectrophotometric assays provided apparentKmandVmaxvalues for active substrates and indicated the applicability of phylogenetic analyses to predict the substrate range of uncharacterized acyl-CoA dehydrogenases. Furthermore, C. necator N-1 was found to utilize 3SP as the sole source of carbon and energy.
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DOI：
10.1073/pnas.90.16.7523
发表时间：
1993-08-15
影响因子：
11.1
作者：
KIM, JJP;WANG, M;PASCHKE, R
通讯作者：
PASCHKE, R
[43]来自埃氏巨球菌的丁酰辅酶A脱氢酶：EC 1.3.99.2丁酰辅酶A：（受体）氧化还原酶
影响因子：
--
作者：
P. Engel
通讯作者：
P. Engel
影响因子：
2.8
作者：
Foerster-Frommne, Karin;Chattopadhyay, Ava;Jendrossek, Dieter
通讯作者：
Jendrossek, Dieter
3-亚磺基丙酸（琥珀酸的类似物）的酶法和化学合成。
DOI：
10.1111/j.1432-1033.1974.tb03346.x
发表时间：
1974
期刊：
European journal of biochemistry
影响因子：
--
作者：
Bernadette Jollès
通讯作者：
Bernadette Jollès
DOI：
--
发表时间：
2011
期刊：
--
影响因子：
--
作者：
Suzanne T Thomas;B. VanderVen;D. Sherman;D. Russell;N. Sampson
通讯作者：
Suzanne T Thomas;B. VanderVen;D. Sherman;D. Russell;N. Sampson