Spectroscopic characterization of oral epithelial dysplasia and squamous cell carcinoma using multiphoton autofluorescence micro-spectroscopy.
Spectroscopic characterization of oral epithelial dysplasia and squamous cell carcinoma using multiphoton autofluorescence micro-spectroscopy.
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DOI：
10.1002/lsm.22697
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发表时间：
2017-11
影响因子：
2.4
通讯作者：
Vargas G
中科院分区：
文献类型：
--
作者：
Pal R;Edward K;Ma L;Qiu S;Vargas G
多光子自体荧光显微镜（MPAM）已显示出潜在的识别功能，直接相关的组织显微结构和生化变化，整个上皮瘤变。在这项研究中，我们评估的自体荧光光谱特性的肿瘤上皮细胞在不典型增生和口腔鳞状细胞癌（OSCC）使用多光子自体荧光光谱（MPAS）在体内仓鼠模型的口腔肿瘤，以确定独特的签名，可用于划定正常口腔粘膜的肿瘤。使用口腔癌前病变和OSCC的9，10-二甲基-1，2-苯并蒽（DMBA）仓鼠模型进行体内MPAM和MPAS。用780 nm激发进行多光子成像和光谱学，而对于MPAM使用450-650 nm的带通发射。通过400-650 nm的光谱窗口收集自体荧光光谱。在780 nm处用荧光激发的MPAS揭示了归因于NADH和FAD的主蓝-绿峰（480-520 nm）的总体红移。在口腔鳞状细胞癌（OSCC）和一些高度异型增生的情况下，在635 nm处观察到归因于PpIX的额外主峰。对照组织和肿瘤组织之间635 nm处的荧光强度以及主要蓝绿峰与635 nm峰的强度比显示出统计学显着差异。已知上皮中的肿瘤转化改变重要组织代谢物如NADH、FAD和PpIX的细胞内稳态，这在其天然环境中通过MPAS观察到。由于多光子激发和深度分辨光谱的更高穿透深度，深层组织显微镜的组合可以被证明是非常宝贵的口腔上皮瘤变的细胞学和生物分子光谱特征的识别。
Multiphoton autofluorescence microscopy (MPAM) has shown potential in identifying features that are directly related to tissue microstructural and biochemical changes throughout epithelial neoplasia. In this study, we evaluate the autofluorescence spectral characteristics of neoplastic epithelium in dysplasia and oral squamous cell carcinoma (OSCC) using multiphoton autofluorescence spectroscopy (MPAS) in an in vivo hamster model of oral neoplasia in order to identify unique signatures that could be used to delineate normal oral mucosa from neoplasia. A 9,10-dimethyl-1,2-benzanthracene (DMBA) hamster model of oral precancer and OSCC was used for in vivo MPAM and MPAS. Multiphoton Imaging and spectroscopy were performed with 780 nm excitation while a bandpass emission 450–650 nm was used for MPAM. Autofluorescence spectra was collected through spectral window of 400–650 nm. MPAS with fluorescence excitation at 780 nm revealed an overall red shift of a primary blue-green peak (480–520 nm) that is attributed to NADH and FAD. In the case of oral squamous cell carcinoma (OSCC) and some high-grade dysplasia an additional prominent peak at 635 nm, attributed to PpIX was observed. The fluorescence intensity at 635 nm and an intensity ratio of the primary blue-green peak vs 635 nm peak, showed statistically significant difference between control and neoplastic tissue. Neoplastic transformation in the epithelium is known to alter the intracellular homeostasis of important tissue metabolites such as NADH, FAD and PpIX, which was observed by MPAS in their native environment. A combination of deep tissue microscopy owing to higher penetration depth of multiphoton excitation and depth resolved spectroscopy could prove to be invaluable in identification of cytologic as well as bio-molecular spectral characteristic of oral epithelial neoplasia.
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影响因子：
3.4
作者：
Palero, Jonathan A.;de Bruijn, Henriette S.;Gerritsen, Hans C.
通讯作者：
Gerritsen, Hans C.
影响因子：
2.8
作者：
Courtnay, Rupert;Ngo, Darleen C.;Karagiannis, Tom C.
通讯作者：
Karagiannis, Tom C.
影响因子：
3.5
作者：
de Veld, DCG;Skurichina, M;Roodenburg, JLN
通讯作者：
Roodenburg, JLN
DOI：
10.1002/path.1700800218
发表时间：
1960-01-01
期刊：
JOURNAL OF PATHOLOGY AND BACTERIOLOGY
影响因子：
--
作者：
GHADIALLY, FN
通讯作者：
GHADIALLY, FN
DOI：
10.1073/pnas.1114746108
发表时间：
2011-10-25
影响因子：
11.1
作者：
Rivera, David R.;Brown, Christopher M.;Xu, Chris
通讯作者：
Xu, Chris