Calcitonin‐like effects of forskolin and choleratoxin on surface area and motility of isolated rabbit osteoclasts
Calcitonin‐like effects of forskolin and choleratoxin on surface area and motility of isolated rabbit osteoclasts
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毛喉素和霍乱毒素对离体兔破骨细胞表面积和运动能力的降钙素样作用
DOI：
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发表时间：
1988
影响因子：
6.2
通讯作者：
J. Heersche
中科院分区：
文献类型：
--
作者：
M. Ransjö;U. Lerner;J. Heersche
我们先前已经发现腺苷环化酶刺激剂Forsklin和霍乱毒素增加了培养的小鼠颅骨的环磷酸腺苷和一过性抑制骨吸收，这表明这些化合物可能直接或间接地抑制破骨细胞的活性。本研究观察了Forsklin和霍乱毒素对兔破骨细胞表面积和运动能力的直接影响，并与降钙素(CT)进行了比较。在没有或存在化合物的情况下，在盖玻片上培养破骨细胞不同时间。在固定和染色的破骨细胞上量化对破骨细胞平均面积的影响，并用时间推移电影显微摄影记录影响。CT(100mU/ml)对平均面积和活动度的影响在几分钟内即可见，并在10-60分钟后最大。福司可林(10-30μ/L)对破骨细胞的运动有快速抑制作用(15-60分钟)，并使破骨细胞收缩面积减小。霍乱毒素(1μg/ml)处理也可引起细胞收缩和运动抑制，但在45-60分钟后才出现反应。Forskolin、CT和霍乱毒素在破骨细胞反应动力学上的差异类似于我们先前报道的对颅骨中环磷酸腺苷的影响的时间进程上的差异。虽然细胞与Forsklin、霍乱毒素或CT连续孵育，但效果是暂时的。在与CT孵育7~8h、Forsklin处理3~4h或霍乱毒素处理4~6h后，破骨细胞开始从收缩和静止中恢复。Forskolin和霍乱毒素对破骨细胞平均表面积的影响呈剂量依赖性。
We have previously found that the adenylate cyclase stimulators forskolin and choleratoxin increase cyclic AMP and transiently inhibit bone resorption in cultured mouse calvaria, suggesting that the compounds, directly or indirectly, may inhibit osteoclast activity. In the present study, forskolin and choleratoxin were investigated for their direct effects on surface area and motility of isolated rabbit osteoclasts, and the effects were compared to those of calcitonin (CT). Osteoclasts were cultured on coverslips for different times in the absence or presence of the compounds. The effect on osteoclast mean area was quantified on fixed and stained osteoclasts, and in addition effects were recorded with time‐lapse cinemicrography. The effects of CT (100 mU/ml) on mean area and motility were seen within minutes and were maximal after 10–60 minutes. Forskolin (10–30 μmol/liter) produced a rapid (15–60 minutes) inhibition of motility and decrease in area (contraction) of osteoclasts. Choleratoxin (1 μg/ml) treatment also resulted in cell contraction and inhibition of motility; however, the response was not seen before 45–60 minutes. The difference in the kinetics of the osteoclast response between forskolin, CT, and choleratoxin is similar to differences in time course for the effect on cyclic AMP in calvarial bones, which we reported earlier. Although cells were incubated continuously with forskolin, choleratoxin, or CT, the effects were transient. Thus, after 7–8 h incubation with CT, 3–4 h treatment with forskolin, or 4–6 h with choleratoxin, the osteoclasts started to recover from contraction and immotility. The effect of forskolin and choleratoxin on the mean surface area of osteoclasts was dose dependent.
毛喉素对器官培养中骨的影响。
DOI：
10.1152/ajpendo.1987.252.1.e44
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
--
作者：
Krieger,NS;Stern,PH
通讯作者：
Stern,PH
降钙素和二丁酰3,5环单磷酸腺苷抑制骨吸收和逃逸的比较。
DOI：
10.1080/07435808109065983
发表时间：
1981
期刊：
Endocrine research communications
影响因子：
--
作者：
McLeod,JF;Raisz,LG
通讯作者：
Raisz,LG
霍乱毒素刺激器官培养中的骨吸收。
DOI：
10.1016/0006-291x(81)91645-4
发表时间：
1981
影响因子：
3.1
作者：
TashjianJr,AH;Ivey,JL
通讯作者：
Ivey,JL