Novel animal model of combined generalized and focal epilepsy.
Novel animal model of combined generalized and focal epilepsy.
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一种新的全身性和局灶性癫痫联合动物模型。
作者：
硫氧还蛋白是一种重要的抗氧化剂，由Txn 1编码，通过调节相互作用蛋白的二巯基/二硫键平衡来保护免受氧化损伤。我们最近发现了Adem大鼠，这是一种携带Txn 1-F54 L突变的癫痫大鼠，其特征是中脑的野生奔跑和空泡变性。本研究旨在描述Adem大鼠癫痫的分类。我们进行了同步视频脑电图记录，磁共振成像，使用气相色谱-质谱（GC-MS）和免疫组织化学进行神经递质测量。Adem大鼠表现出失神、强直和局灶性癫痫发作。癫痫的类型被分类为全身性和局灶性癫痫相结合。在3周龄时测量中脑和皮质中的神经递质，此时中脑中发生神经元细胞死亡。GC-MS的结果排除了Adem大鼠中脑和皮质中兴奋系统的优势。星形胶质细胞和小胶质细胞的激活在5周龄时更明显，此时癫痫发作频繁发生。Adem大鼠的基础病理学仍未知。然而，胶质细胞活化和炎症可能在癫痫的发生中起重要作用。
Thioredoxin, encoded by Txn1, is a critical antioxidant that protects against oxidative damage by regulating the dithiol/disulfide balance of interacting proteins. We recently discovered the Adem rat, an epileptic rat harboring the Txn1‐F54L mutation, characterized by wild running and vacuolar degeneration in the midbrain. This study aimed to characterize the classification of epilepsy in Adem rats. We performed simultaneous video‐electroencephalographic recordings, magnetic resonance imaging, neurotransmitter measurements using gas chromatography–mass spectrometry (GC‐MS), and immunohistochemistry. Adem rats exhibited absence, tonic, and focal seizures. The type of epilepsy was classified as combined generalized and focal epilepsy. Neurotransmitters in the midbrain and cortex were measured at 3 weeks of age, when neuronal cell death occurs in the midbrain. The results of GC‐MS ruled out the dominance of the excitatory system in the midbrain and cortex of Adem rats. Activation of astrocytes and microglia was more pronounced at 5 weeks of age, at which time epileptic seizures occurred frequently. The underlying pathology in Adem rats remains unknown. However, glial cell activation and inflammation may play a significant role in the occurrence of epilepsy.
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