Deuterated water imaging of the rat brain following metabolism of [(2) H(7) ]glucose.
Deuterated water imaging of the rat brain following metabolism of [(2) H(7) ]glucose.
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[（2）H（7）]葡萄糖代谢后大鼠脑的氘水成像。
DOI：
10.1002/mrm.28700
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发表时间：
2021-06
影响因子：
3.3
通讯作者：
Merritt ME
中科院分区：
文献类型：
--
作者：
Mahar R;Zeng H;Giacalone A;Ragavan M;Mareci TH;Merritt ME
确定[2 H7]葡萄糖代谢产生的HDO是否是脑糖酵解和氧化通量的敏感生物标志物。通过尾静脉向Sprague-Dawley大鼠注射1.95 g/kg的[2 H7]葡萄糖推注。将2 H表面线圈放置在头顶上以每1.3分钟记录脑的2 H光谱，以测量葡萄糖摄取和代谢为HDO、乳酸盐和谷氨酸盐/谷氨酰胺。采用基于梯度回波序列的两点狄克逊法选择性重建氘代葡萄糖和水（HDO）图像。背景HDO信号可以在葡萄糖注射之前被检测和成像。2 H NMR谱显示[2 H7]葡萄糖的到达及其以时间依赖性方式的代谢。HDO与谷氨酸/谷氨酰胺（glx）共振的比率证明了注射后的伪稳态，其中脑代谢主导由外周代谢产生的HDO的洗入。脑光谱学揭示，HDO生成与适当的伪稳态窗口中的乳酸盐和glx外观呈线性。HDO和葡萄糖的选择性成像很容易使用梯度回波方法完成。HDO的代谢成像，作为葡萄糖，乳酸和glx代谢的标志物，已在这里首次显示。使用标准梯度回波序列可以有效地评估脑葡萄糖代谢，与基于化学位移成像的技术相比，该标准梯度回波序列提供了上级平面分辨率。
To determine if HDO generated from metabolism of [2H7]glucose is a sensitive biomarker of cerebral glycolysis and oxidative flux. A bolus of [2H7]glucose was injected via tail vein at 1.95 g/kg into Sprague-Dawley rats. A 2H surface coil was placed on top of the head to record 2H spectra of the brain every 1.3 minutes to measure glucose uptake and metabolism to HDO, lactate, and glutamate/glutamine. A two-point Dixon method based on a gradient-echo sequence was used to reconstruct deuterated glucose and water (HDO) images selectively. The background HDO signal could be detected and imaged prior to glucose injection. 2H NMR spectra showed arrival of [2H7]glucose and its metabolism in a time dependent manner. A ratio of the HDO to glutamate/glutamine (glx) resonances demonstrates a pseudo-steady state following injection where cerebral metabolism dominates wash-in of HDO generated by peripheral metabolism. Brain spectroscopy reveals that HDO generation is linear with lactate and glx appearance in the appropriate pseudo-steady state window. Selective imaging of HDO and glucose is easily accomplished using a gradient echo method. Metabolic imaging of HDO, as a marker of glucose, lactate, and glx metabolism, has been shown here for the first-time. Cerebral glucose metabolism can be assessed efficiently using a standard gradient echo sequence that provides superior in plane resolution compared to chemical shift imaging based techniques.
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影响因子：
5.9
作者：
Salamanca-Cardona L;Keshari KR
通讯作者：
Keshari KR
影响因子：
5.7
作者：
Villien M;Wey HY;Mandeville JB;Catana C;Polimeni JR;Sander CY;Zürcher NR;Chonde DB;Fowler JS;Rosen BR;Hooker JM
通讯作者：
Hooker JM
影响因子：
2.2
作者：
FRAHM, J;MERBOLDT, KD;HAASE, A
通讯作者：
HAASE, A
影响因子：
5.5
作者：
Shreve, PD;Anzai, Y;Wahl, RL
通讯作者：
Wahl, RL
影响因子：
6.3
作者：
Lu, Ming;Zhu, Xiao-Hong;Chen, Wei
通讯作者：
Chen, Wei