Regulatory Networks Governing Methionine Catabolism into Volatile Organic Sulfur-Containing Compounds in Clonostachys rosea
Regulatory Networks Governing Methionine Catabolism into Volatile Organic Sulfur-Containing Compounds in Clonostachys rosea
复制标题
控制红竹中蛋氨酸分解代谢为挥发性有机含硫化合物的监管网络
DOI：
10.1128/aem.01840-18
复制
发表时间：
2018-09
影响因子：
4.4
通讯作者：
Tang Ya Jie
中科院分区：
文献类型：
--
作者：
Xu Yang Hua;Jia Kai Zhi;Tang Ya Jie
甲烷是有机氮的转运体，在氮代谢中起着重要作用。外源Met强烈诱导ARO 8 -2和PDC的表达，抑制STR 3的表达，并通过埃利希和脱甲硫醚途径产生挥发性有机硫化合物。在这项研究中，我们使用遗传，生物信息学和代谢物为基础的分析，以确认转录控制的氨基转移酶基因ARO 8 -2，脱羧酶基因PDC和脱甲硫醚酶基因STR 3调制成挥发性有机含硫化合物的蛋氨酸催化剂。重要的是，我们发现，除了埃利希途径，脱甲醇途径的氮代谢抑制敏感的调节途径，控制基因的转录需要分解代谢穷人的氮源进行调节。这项工作显着推进了我们的理解氮代谢产物抑制敏感的转录调控含硫氨基酸催化剂，并提供了一个基础工程蛋氨酸催化剂途径的生产燃料和有价值的风味醇。适应环境扰动需要生命系统协调调节信号通路、基因表达和代谢。为了更好地理解适应的机制，必须阐明网络中的调节节点。在这里，ARO 8 -2（编码氨基转移酶），PDC（编码脱羧酶），和STR 3（编码脱甲硫醇酶）被确定为关键基因参与甲硫氨酸在真菌寄生真菌Clonostachys rosea，分离自块茎黑孢子囊果。外源Met诱导ARO 8 -2和PDC的转录，但抑制STR 3的转录，这是由推测的MSN 2和GLN 3结合位点响应氮分解代谢物阻遏控制的。Met及其结构衍生物作为谷氨酰胺合成酶抑制剂发挥作用，导致STR 3表达下调。推测的GLN 3结合位点是STR 3下调所必需的。在酿酒酵母中，Met及其结构衍生物也引发了去甲硫醇酶基因表达的下调。结果表明，外源蛋氨酸通过抑制氮代谢产物的代谢，刺激埃利希途径，并通过蛋氨酸亚砜亚胺反应性调节途径对脱甲硫氨酸途径进行负调控。这一发现揭示了网络中控制Met转化为挥发性有机含硫化合物的调控节点，从而增强了我们对适应的理解。重要性蛋氨酸穿梭有机氮，并在氮代谢中发挥核心作用。外源Met强烈诱导ARO 8 -2和PDC的表达，抑制STR 3的表达，并通过埃利希和脱甲硫醚途径产生挥发性有机硫化合物。在这项研究中，我们使用遗传，生物信息学和代谢物为基础的分析，以确认转录控制的氨基转移酶基因ARO 8 -2，脱羧酶基因PDC和脱甲硫醚酶基因STR 3调制成挥发性有机含硫化合物的蛋氨酸催化剂。重要的是，我们发现，除了埃利希途径，脱甲醇途径的氮代谢抑制敏感的调节途径，控制基因的转录需要分解代谢穷人的氮源进行调节。这项工作显着推进了我们的理解氮代谢产物抑制敏感的转录调控含硫氨基酸催化剂，并提供了一个基础工程蛋氨酸催化剂途径的生产燃料和有价值的风味醇。
Methionine shuttles organic nitrogen and plays a central role in nitrogen metabolism. Exogenous Met strongly induces the expression of ARO8-2 and PDC, represses the expression of STR3, and generates volatile organic sulfur-containing compounds via the Ehrlich and demethiolation pathways. In this study, we used genetic, bioinformatic, and metabolite-based analyses to confirm that transcriptional control of the aminotransferase gene ARO8-2, the decarboxylase gene PDC, and the demethiolase gene STR3 modulates Met catabolism into volatile organic sulfur-containing compounds. Importantly, we found that, in addition to the Ehrlich pathway, the demethiolation pathway was regulated by a nitrogen catabolite repression-sensitive regulatory pathway that controlled the transcription of genes required to catabolize poor nitrogen sources. This work significantly advances our understanding of nitrogen catabolite repression-sensitive transcriptional regulation of sulfur-containing amino acid catabolism and provides a basis for engineering Met catabolism pathways for the production of fuel and valuable flavor alcohols. ABSTRACT Adaptation to environmental perturbations requires living systems to coordinately regulate signaling pathways, gene expression, and metabolism. To better understand the mechanisms underlying adaptation, the regulatory nodes within networks must be elucidated. Here, ARO8-2 (which encodes an aminotransferase), PDC (which encodes a decarboxylase), and STR3 (which encodes a demethiolase) were identified as key genes involved in the catabolism of methionine in the mycoparasitic fungus Clonostachys rosea, isolated from Tuber melanosporum ascocarps. Exogenous Met induced the transcription of ARO8-2 and PDC but repressed the transcription of STR3, which is controlled by the putative MSN2 and GLN3 binding sites responding to nitrogen catabolite repression. Met and its structural derivatives function as glutamine synthetase inhibitors, resulting in the downregulation of STR3 expression. The putative GLN3 binding site was necessary for STR3 downregulation. In Saccharomyces cerevisiae, Met and its structural derivatives also triggered downregulation of demethiolase gene expression. Altogether, the results indicated that exogenous Met triggered nitrogen catabolite repression, which stimulated the Ehrlich pathway and negatively regulated the demethiolation pathway via the methionine sulfoximine-responsive regulatory pathway. This finding revealed the regulatory nodes within the networks controlling the catabolism of Met into volatile organic sulfur-containing compounds, thereby enhancing our understanding of adaptation. IMPORTANCE Methionine shuttles organic nitrogen and plays a central role in nitrogen metabolism. Exogenous Met strongly induces the expression of ARO8-2 and PDC, represses the expression of STR3, and generates volatile organic sulfur-containing compounds via the Ehrlich and demethiolation pathways. In this study, we used genetic, bioinformatic, and metabolite-based analyses to confirm that transcriptional control of the aminotransferase gene ARO8-2, the decarboxylase gene PDC, and the demethiolase gene STR3 modulates Met catabolism into volatile organic sulfur-containing compounds. Importantly, we found that, in addition to the Ehrlich pathway, the demethiolation pathway was regulated by a nitrogen catabolite repression-sensitive regulatory pathway that controlled the transcription of genes required to catabolize poor nitrogen sources. This work significantly advances our understanding of nitrogen catabolite repression-sensitive transcriptional regulation of sulfur-containing amino acid catabolism and provides a basis for engineering Met catabolism pathways for the production of fuel and valuable flavor alcohols.
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影响因子：
11.3
作者：
Conrad M;Schothorst J;Kankipati HN;Van Zeebroeck G;Rubio-Texeira M;Thevelein JM
通讯作者：
Thevelein JM
影响因子：
16
作者：
Ndoja, Ada;Cohen, Robert E.;Yao, Tingting
通讯作者：
Yao, Tingting
DOI：
10.1016/0005-2736(72)90146-0
发表时间：
1972-04
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
F. Meins;Marc L. Abrams
通讯作者：
F. Meins;Marc L. Abrams
影响因子：
4.3
作者：
Eden E;Lipson D;Yogev S;Yakhini Z
通讯作者：
Yakhini Z
影响因子：
2.9
作者：
RONZIO, RA;ROWE, WB;MEISTER, A
通讯作者：
MEISTER, A