Comparative genomics and transcriptomics of Propionibacterium acnes.
Comparative genomics and transcriptomics of Propionibacterium acnes.
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DOI：
10.1371/journal.pone.0021581
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Brüggemann H
中科院分区：
文献类型：
--
作者：
Brzuszkiewicz E;Weiner J;Wollherr A;Thürmer A;Hüpeden J;Lomholt HB;Kilian M;Gottschalk G;Daniel R;Mollenkopf HJ;Meyer TF;Brüggemann H
厌氧革兰氏阳性细菌痤疮丙酸杆菌是一种偶尔与炎症性疾病有关的人类皮肤真菌。最近的研究表明，痤疮丙酸杆菌的进化不同谱系在疾病中起病因作用，而其他谱系与维持皮肤稳态有关。为了阐明差异菌株特性的分子基础，我们对不同的痤疮丙酸杆菌菌株进行了基因组和转录组学分析。我们对痤疮丙酸杆菌菌株266（一种I-1a型菌株）的基因组进行了测序。菌株266和其他四种痤疮丙酸杆菌菌株的比较基因组分析显示，总体基因组可塑性相对较低;然而，根据对痤疮丙酸杆菌菌株集合的PCR分析判断，许多岛状基因组区域编码各种推定的毒力相关和适应性性状，这些基因组区域在不同的痤疮类型之间存在差异。比较转录组分析的菌株KPA 171202（I-2型）和266在指数增长过程中揭示了运输系统和代谢途径的基因表达菌株间的差异。此外，转录水平的基因编码可能的毒力因子，如皮肤素硫酸盐粘附素，多不饱和脂肪酸异构酶，铁收购蛋白HtaA和脂肪酶GehA的上调菌株266。我们研究了指数生长期和稳定生长期的差异基因表达。编码节能呼吸链以及分泌和毒力相关因子的基因在指数期被转录，而静止生长期的特征在于参与应激反应和氨基酸代谢的基因上调。我们的数据突出了菌株多样性的基因组基础，并首次鉴定了基因组的活跃转录部分，强调了生长状态在痤疮丙酸杆菌的炎症诱导活性中发挥的重要作用。我们认为，不同的痤疮丙酸杆菌菌株的致病潜力不仅取决于特定类型的基因组含量，但也通过可变的基因表达。
The anaerobic Gram-positive bacterium Propionibacterium acnes is a human skin commensal that is occasionally associated with inflammatory diseases. Recent work has indicated that evolutionary distinct lineages of P. acnes play etiologic roles in disease while others are associated with maintenance of skin homeostasis. To shed light on the molecular basis for differential strain properties, we carried out genomic and transcriptomic analysis of distinct P. acnes strains. We sequenced the genome of the P. acnes strain 266, a type I-1a strain. Comparative genome analysis of strain 266 and four other P. acnes strains revealed that overall genome plasticity is relatively low; however, a number of island-like genomic regions, encoding a variety of putative virulence-associated and fitness traits differ between phylotypes, as judged from PCR analysis of a collection of P. acnes strains. Comparative transcriptome analysis of strains KPA171202 (type I-2) and 266 during exponential growth revealed inter-strain differences in gene expression of transport systems and metabolic pathways. In addition, transcript levels of genes encoding possible virulence factors such as dermatan-sulphate adhesin, polyunsaturated fatty acid isomerase, iron acquisition protein HtaA and lipase GehA were upregulated in strain 266. We investigated differential gene expression during exponential and stationary growth phases. Genes encoding components of the energy-conserving respiratory chain as well as secreted and virulence-associated factors were transcribed during the exponential phase, while the stationary growth phase was characterized by upregulation of genes involved in stress responses and amino acid metabolism. Our data highlight the genomic basis for strain diversity and identify, for the first time, the actively transcribed part of the genome, underlining the important role growth status plays in the inflammation-inducing activity of P. acnes. We argue that the disease-causing potential of different P. acnes strains is not only determined by the phylotype-specific genome content but also by variable gene expression.
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影响因子：
10.3
作者：
Jappe, U;Ingham, E;Holland, KT
通讯作者：
Holland, KT
影响因子：
3.2
作者：
Jorgensen, Mikkel G.;Pandey, Deo P.;Gerdes, Kenn
通讯作者：
Gerdes, Kenn
DOI：
10.1073/pnas.0900008105
发表时间：
2009-02-24
影响因子：
11.1
作者：
Brown, Laura C. Wieland;Acker, Michael G.;Fischbach, Michael A.
通讯作者：
Fischbach, Michael A.
影响因子：
10.3
作者：
Jugeau, S;Tenaud, I;Dreno, B
通讯作者：
Dreno, B
影响因子：
56.9
作者：
Brüggemann, H;Henne, A;Gottschalk, G
通讯作者：
Gottschalk, G