Metabolic phenotypes of standard and cold-stored platelets.
Metabolic phenotypes of standard and cold-stored platelets.
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DOI：
10.1111/trf.15651
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发表时间：
2020-06
期刊：
影响因子：
2.9
通讯作者：
Spinella PC
中科院分区：
文献类型：
--
作者：
D'Alessandro A;Thomas KA;Stefanoni D;Gamboni F;Shea SM;Reisz JA;Spinella PC
传统的血小板（PLT）在室温下连续搅拌储存导致有限的保质期（5天）和细菌污染的风险增加。然而，这两个方面都可以通过冷藏来改善。初步研究表明，与室温保存的plt相比，plt可以冷藏长达3周，同时保持其代谢活性的时间更长。因此，在本研究中，我们假设在4°C保存3周的plt的代谢表型可以与在22°C室温保存5天的plt相媲美。对在室温（22°C）下保存5天或在冷藏条件（4°C）下保存3周的9个单采PLT浓缩物进行代谢组学分析。冷藏不影响谷氨酰胺的下降速率或克雷布斯循环代谢物上游富马酸盐和苹果酸盐的细胞内水平。然而，它确实降低了氧化应激（对谷胱甘肽和嘌呤），减缓了戊糖磷酸途径、糖酵解和脂肪酸代谢（酰基肉碱）的激活。4°C plt在第10天的整体代谢表型与在22°C保存的plt在5天保质期结束时的代谢表型相当，而糖酵解、嘌呤和脂肪酸代谢的额外变化在第21天被注意到。
Conventional platelet (PLT) storage at room temperature under continuous agitation results in a limited shelf life (5 days) and an increased risk of bacterial contamination. However, both of these aspects can be ameliorated by cold storage. Preliminary work has suggested that PLTs can be cold stored for up to 3 weeks, while preserving their metabolic activity longer than in PLTs stored at room temperature. As such, in the present study, we hypothesized that the metabolic phenotypes of PLTs stored at 4°C for 3 weeks could be comparable to that of room temperature–stored PLTs at 22°C for 5 days. Metabolomics analyses were performed on nine apheresis PLT concentrates stored either at room temperature (22°C) for 5 days or refrigerated conditions (4°C) for up to 3 weeks. Refrigeration did not impact the rate of decline in glutamine or the intracellular levels of Krebs cycle metabolites upstream to fumarate and malate. It did, however, decrease oxidant stress (to glutathione and purines) and slowed down the activation of the pentose phosphate pathway, glycolysis, and fatty acid metabolism (acyl-carnitines). The overall metabolic phenotypes of 4°C PLTs at Storage Day 10 are comparable to PLTs stored at 22°C at the end of their 5-day shelf life, while additional changes in glycolysis, purine, and fatty acid metabolism are noted by Day 21.
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血小板功能的粘弹性测量，而不是纤维蛋白原功能可以预测创伤患者中对组织型纤溶酶原激活剂的敏感性。
DOI：
10.1111/jth.13067
发表时间：
2015-10
期刊：
Journal of thrombosis and haemostasis : JTH
影响因子：
--
作者：
Moore HB;Moore EE;Chapman MP;Gonzalez E;Slaughter AL;Morton AP;D'Alessandro A;Hansen KC;Sauaia A;Banerjee A;Silliman CC
通讯作者：
Silliman CC
影响因子：
2.9
作者：
Marrocco, Cristina;D'Alessandro, Angelo;Zolla, Lello
通讯作者：
Zolla, Lello
影响因子：
2.7
作者：
Badlou, B. A.;van der Meer, P. F.;Pietersz, R. N. I.
通讯作者：
Pietersz, R. N. I.
影响因子：
20.3
作者：
MANNO, CS;HEDBERG, KW;NORWOOD, WI
通讯作者：
NORWOOD, WI
DOI：
10.1016/j.biocel.2005.04.014
发表时间：
2005-09-01
影响因子：
4
作者：
Homma, R;Mase, A;Igarashi, K
通讯作者：
Igarashi, K