The WAVE/SCAR complex promotes polarized cell movements and actin enrichment in epithelia during C. elegans embryogenesis.
The WAVE/SCAR complex promotes polarized cell movements and actin enrichment in epithelia during C. elegans embryogenesis.
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DOI：
10.1016/j.ydbio.2008.09.023
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发表时间：
2008-12-15
影响因子：
2.7
通讯作者：
Soto, Martha C.
中科院分区：
文献类型：
--
作者：
Patel, Falshruti B.;Bernadskaya, Yelena Y.;Chen, Esteban;Jobanputra, Aesha;Pooladi, Zahra;Freeman, Kristy L.;Gally, Christelle;Mohler, William A.;Soto, Martha C.
WAVE/SCAR复合体通过Arp2/3复合体促进肌动蛋白的成核，以响应RAC信号。我们发现，线虫WVE-1/Gex-1是线虫Wave/SCAR的唯一同源物，通过基因突变或RNAi缺失，与线虫Wave/SCAR复合体的另外三个成分Gex-2/Sra1/p140/PIR121、Gex-3/NAP1/HEM2/Kette或ABI-1/ABI的丢失具有相同的表型。我们发现，整个波/疤痕复合体在发育过程中的不同时间和不同组织中促进了肌动蛋白依赖的事件。在线虫胚胎发生过程中失去CED-10/rac1，WAVE/SCAR复合体成分或Arp2/3阻止了表皮细胞的迁移，尽管表皮细胞分化正确。4D影片显示，这种失效是由于特定的表皮细胞膜动力学降低所致。与果蝇的成肌细胞不同，线虫的表皮细胞融合可以在没有WAVE/SCAR或Arp2/3的情况下发生。相反，我们发现上皮组织中F-肌动蛋白的亚细胞浓缩需要Rac-WAVE/SCAR-Arp2/3途径。有趣的是，我们发现在发育的同一阶段，F-肌动蛋白和WAVE/SCAR蛋白都在一种上皮组织的顶部和另一种上皮组织的底部富含。我们认为，在形态发生过程中，RAC-WAVE/SCAR-Arp2/3通路在时间和空间上调节肌动蛋白的核化是上皮细胞组织和运动所必需的。
The WAVE/SCAR complex promotes actin nucleation through the Arp2/3 complex, in response to Rac signaling. We show that loss of WVE-1/GEX-1, the only C. elegans WAVE/SCAR homolog, by genetic mutation or by RNAi, has the same phenotype as loss of GEX-2/Sra1/p140/PIR121, GEX-3/NAP1/HEM2/KETTE, or ABI-1/ABI, the three other components of the C. elegans WAVE/SCAR complex. We find that the entire WAVE/SCAR complex promotes actin-dependent events at different times and in different tissues during development. During C. elegans embryogenesis loss of CED-10/Rac1, WAVE/SCAR complex components, or Arp2/3 blocks epidermal cell migrations despite correct epidermal cell differentiation. 4D movies show that this failure occurs due to decreased membrane dynamics in specific epidermal cells. Unlike myoblasts in Drosophila, epidermal cell fusions in C. elegans can occur in the absence of WAVE/SCAR or Arp2/3. Instead we find that subcellular enrichment of F-actin in epithelial tissues requires the Rac-WAVE/SCAR- Arp2/3 pathway. Intriguingly, we find that at the same stage of development both F-actin and WAVE/SCAR proteins are enriched apically in one epithelial tissue and basolaterally in another. We propose that temporally and spatially regulated actin nucleation by the Rac-WAVE/SCAR- Arp2/3 pathway is required for epithelial cell organization and movements during morphogenesis.
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DOI：
10.1083/jcb.146.3.659
发表时间：
1999-08-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hresko MC;Schriefer LA;Shrimankar P;Waterston RH
通讯作者：
Waterston RH
DOI：
10.1083/jcb.101.4.1532
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Francis GR;Waterston RH
通讯作者：
Waterston RH
影响因子：
4.6
作者：
Frank, MJ;Cartwright, HN;Smith, LG
通讯作者：
Smith, LG
影响因子：
7.8
作者：
Bosher, Julia M;Hahn, Bum-Soo;Legouis, Renaud;Sookhareea, Satis;Weimer, Robby M;Gansmuller, Anne;Chisholm, Andrew D;Rose, Ann M;Bessereau, Jean-Louis;Labouesse, Michel
通讯作者：
Labouesse, Michel
影响因子：
3.3
作者：
Firestein, BL;Rongo, C
通讯作者：
Rongo, C