Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs.
Comparison of the osteogenic potential of mesenchymal stem cells from the bone marrow and adipose tissue of young dogs.
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DOI：
10.1186/s12917-014-0190-y
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发表时间：
2014-09-02
影响因子：
2.6
通讯作者：
Rezende CM
中科院分区：
文献类型：
--
作者：
Alves EG;Serakides R;Boeloni JN;Rosado IR;Ocarino NM;Oliveira HP;Góes AM;Rezende CM
本研究的目的是比较从幼犬的骨髓（BM-MSCs）和脂肪组织（AD-MSCs）中提取的间充质干细胞的成骨潜能。评估了以下参数：二甲基噻唑基联苯四氮唑（MTT）转化率、碱性磷酸酶（ALP）活性、胶原蛋白和矿化基质合成以及osterix、骨唾液蛋白（BSP）和骨钙素（OC）的表达。在分化14和21天后，BM-MSC中的MTT转化率高于AD-MSC;分化的AD-MSC中的ALP活性在第7天更高; BM-MSC中的胶原合成在第14和21天更高; BM-MSC中的每视野矿化面积百分比高于AD-MSC;在第14天和第21天，osterix在BM-MSCs中的表达更高，并且在所有研究时间点，BSP和OC在BM-MSCs中的表达水平更高。结论：从幼犬中提取的BM-MSCs的成骨潜能大于AD-MSCs。
The aim of the present study was to compare the osteogenic potential of mesenchymal stem cells extracted from the bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) of young dogs. The following parameters were assessed: dimethyl thiazolyl diphenyl tetrazolium (MTT) conversion, alkaline phosphatase (ALP) activity, collagen and mineralised matrix synthesis, and the expressions of osterix, bone sialoprotein (BSP), and osteocalcin (OC). MTT conversion was greater in BM-MSCs compared to AD-MSCs after 14 and 21 days of differentiation; ALP activity was greater in differentiated AD-MSCs on day 7; collagen synthesis was greater in BM-MSCs on days 14 and 21; the percentage of mineralized area per field was greater in BM-MSCs compared to AD-MSCs; osterix expression was greater in BM-MSCs in days 14 and 21, and BSP and OC expression levels were greater in BM-MSCs at all the investigation time-points. It was concluded that the osteogenic potential was greater in BM-MSCs than AD-MSCs when extracted from young dogs.
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影响因子：
4.3
作者：
Lee, Won Sik;Suzuki, Yasuhiro;Graves, Scott S.;Iwata, Mineo;Venkataraman, G. M.;Mielcarek, Marco;Peterson, Laura J.;Ikehara, Susumu;Torok-Storb, Beverly;Storb, Rainer
通讯作者：
Storb, Rainer
影响因子：
6.3
作者：
Hell, R. C. R.;Ocarino, N. M.;Serakides, R.
通讯作者：
Serakides, R.
影响因子：
2.7
作者：
Franceschi, Renny T.;Ge, Chunxi;Jiang, Di
通讯作者：
Jiang, Di
影响因子：
64.5
作者：
Nakashima, K;Zhou, X;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
3.5
作者：
Zaim, Merve;Karaman, Serap;Isik, Sevim
通讯作者：
Isik, Sevim