Application and analysis of the folin ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L.
Application and analysis of the folin ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L.
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DOI：
10.3390/molecules18066852
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发表时间：
2013-06-10
期刊：
影响因子：
--
通讯作者：
de Mello JC
中科院分区：
文献类型：
--
作者：
Blainski A;Lopes GC;de Mello JC
巴西补血草（Limonium brasiliense）是巴西南部海岸常见的植物。根传统上用于治疗经前综合征，月经失调和生殖器泌尿感染。从其根中获得并用于这些目的的药物制剂于20世纪80年代和90年代在巴西上市。目前，巴西药品管理局（国家卫生监督局，ANVISA）已经取消了这些产品的注册，并且由于缺乏研究来表征植物原料并确保其使用的有效性和安全性，因此停止使用这些产品。本研究的目的是建立和验证一种分析方法来测定提取物中的总多酚（TP）的含量。采用紫外/维斯分光光度法测定了巴西甘草根的总黄酮含量。L.在丙酮：水（7：3，v/v-10%w/v）中提取巴西根。用粗提物建立了茶多酚的含量测定方法。根据国家和国际指南对该方法进行了验证。最佳分析时间为30 min，最佳波长为760 nm，最佳标准物质为邻苯三酚。在这些条件下，通过UV/维斯分光光度法进行的验证证明，该方法具有线性、专属性、精密度、准确度、重现性、耐用性和易操作性。该方法符合分析应用的要求，并确保结果的可靠性。
Limonium brasiliense is a common plant on the southern coast of Brazil. The roots are traditionally used for treatment of premenstrual syndrome, menstrual disturbances and genito-urinary infections. Pharmaceutical preparations obtained from its roots and used for these purposes were marketed in Brazil in the 1980s and 1990s. Currently, the Brazilian Drug Agency (National Health Surveillance Agency, ANVISA) has canceled the registration of these products, and their use was discontinued because of a lack of studies to characterize the plant raw material and ensure the effectiveness and safety of its use. The aim of the present study was to develop and validate an analytical method to determine the content of total polyphenols (TP) in an extract from L. brasiliense roots, by the UV/Vis spectrophotometric method. L. brasiliense roots were extracted in acetone:water (7:3, v/v-10% w/v). The crude extract was used to develop a method for TP assay. The method was validated according to national and international guidelines. The optimum conditions for analysis time, wavelength, and standard substance were 30 min, 760 nm, and pyrogallol, respectively. Under these conditions, validation by UV/Vis spectrophotometry proved the method to be linear, specific, precise, accurate, reproducible, robust, and easy to perform. This methodology complies with the requirements for analytical application and to ensure the reliability of the results.
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影响因子：
4.8
作者：
Cicco, Nunzia;Lanorte, Maria T.;Lattanzio, Vincenzo
通讯作者：
Lattanzio, Vincenzo
影响因子：
3.8
作者：
Ferreira, D;Marais, JPJ;Slade, D
通讯作者：
Slade, D
DOI：
10.1590/s0100-40422004000500017
发表时间：
2004-10-01
期刊：
Química Nova
影响因子：
--
作者：
Ribani, Marcelo;Bottoli, Carla Beatriz Grespan;Melo, Lúcio Flávio Costa
通讯作者：
Melo, Lúcio Flávio Costa
影响因子：
8.8
作者：
Gülçin, I;Sat, IG;Küfrevioglu, OI
通讯作者：
Küfrevioglu, OI
影响因子：
2.8
作者：
Gottlieb, OR;Borin, MRD
通讯作者：
Borin, MRD