Genetic variation in CaTIFY4b contributes to drought adaptation in chickpea.
Genetic variation in CaTIFY4b contributes to drought adaptation in chickpea.
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DOI：
10.1111/pbi.13840
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发表时间：
2022-09
影响因子：
13.8
通讯作者：
Varshney, Rajeev K.
中科院分区：
文献类型：
--
作者：
Barmukh, Rutwik;Roorkiwal, Manish;Garg, Vanika;Khan, Aamir W.;German, Liam;Jaganathan, Deepa;Chitikineni, Annapurna;Kholova, Jana;Kudapa, Himabindu;Sivasakthi, Kaliamoorthy;Samineni, Srinivasan;Kale, Sandip M.;Gaur, Pooran M.;Sagurthi, Someswar Rao;Benitez-Alfonso, Yoselin;Varshney, Rajeev K.
鹰嘴豆生产易受干旱胁迫的影响。确定干旱适应的遗传成分对于提高鹰嘴豆产量至关重要。在这里，我们提出了“QTL热点”的精细定位和表征，这是一个控制鹰嘴豆生长的基因组区域，对干旱条件下的作物生产具有积极影响。我们报告了转录因子CaTIFY4b中的非同义取代调节鹰嘴豆的种子重量和器官大小。CaTIFY4b在蒺藜苜蓿中的异位表达增强了水分亏缺下的根系生长。我们的研究结果表明，“QTL热点”中的等位基因变异改善了花前水分利用、蒸腾效率、根系构型和冠层发育，从而在终端干旱条件下实现高产表现。基因表达分析表明，CaTIFY4b可能通过调节生长调节因子的转录共激活因子GRF相互作用因子1（GIF 1）的表达来调节水分亏缺下的器官大小。总之，我们的研究为CaTIFY4b的作用以及在特定干旱情况下支撑鹰嘴豆生长和生产的各种生理和分子机制提供了新的见解。
Chickpea production is vulnerable to drought stress. Identifying the genetic components underlying drought adaptation is crucial for enhancing chickpea productivity. Here, we present the fine mapping and characterization of ‘QTL‐hotspot’, a genomic region controlling chickpea growth with positive consequences on crop production under drought. We report that a non‐synonymous substitution in the transcription factor CaTIFY4b regulates seed weight and organ size in chickpea. Ectopic expression of CaTIFY4b in Medicago truncatula enhances root growth under water deficit. Our results suggest that allelic variation in ‘QTL‐hotspot’ improves pre‐anthesis water use, transpiration efficiency, root architecture and canopy development, enabling high‐yield performance under terminal drought conditions. Gene expression analysis indicated that CaTIFY4b may regulate organ size under water deficit by modulating the expression of GRF‐INTERACTING FACTOR1 (GIF1), a transcriptional co‐activator of Growth‐Regulating Factors. Taken together, our study offers new insights into the role of CaTIFY4b and on diverse physiological and molecular mechanisms underpinning chickpea growth and production under specific drought scenarios.
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影响因子：
4.2
作者：
Bharadwaj, Chellapilla;Tripathi, Shailesh;Varshney, Rajeev K.
通讯作者：
Varshney, Rajeev K.
影响因子：
11.6
作者：
Chung, Hoo Sun;Howe, Gregg A.
通讯作者：
Howe, Gregg A.
影响因子：
3.5
作者：
QUANDT, HJ;PUHLER, A;BROER, I
通讯作者：
BROER, I
影响因子：
7.3
作者：
Kudapa, Himabindu;Garg, Vanika;Varshney, Rajeev K.
通讯作者：
Varshney, Rajeev K.
DOI：
10.1007/s00438-014-0932-3
发表时间：
2015-04
期刊：
Molecular genetics and genomics : MGG
影响因子：
--
作者：
Jaganathan D;Thudi M;Kale S;Azam S;Roorkiwal M;Gaur PM;Kishor PB;Nguyen H;Sutton T;Varshney RK
通讯作者：
Varshney RK