Heterologous expression of human costimulatory molecule B7-2 and construction of B7-2 immobilized polyhydroxyalkanoate nanoparticles for use as an immune activation agent.
Heterologous expression of human costimulatory molecule B7-2 and construction of B7-2 immobilized polyhydroxyalkanoate nanoparticles for use as an immune activation agent.
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人共刺激分子B7-2的异源表达及用作免疫激活剂的B7-2固定化聚羟基脂肪酸酯纳米粒子的构建
DOI：
10.1186/1472-6750-12-43
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发表时间：
2012-07-30
影响因子：
3.5
通讯作者：
Wang LL
中科院分区：
文献类型：
--
作者：
Li MC;Liu QQ;Lu XY;Zhang YL;Wang LL
Costimulation of T cells via costimulatory molecules such as B7 is important for eliciting cell-mediated antitumor immunity. Presenting costimulation molecules by immobilizing recombinant B7 on the surface of nanovectors is a novel strategy for complementary therapy. Polyhydroxyalkanoates (PHAs) are a family of biodegradable, non-toxic, biocompatible polyesters, which can be used as a nonspecific immobilizing matrix for protein presentation. Recombinant protein fusion with PHA granule binding protein phasin (PhaP) can be easily immobilized on the surface of PHA nanoparticles through hydrophobic interactions between PhaP and PHA, and therefore provides a low-cost protein presenting strategy. In this study, the extracellular domain of the B7-2 molecule (also named as CD86) was fused with PhaP at its N-terminal and heterogeneously expressed in recombinant Escherichia coli strain BL21 (DE3). The purified B7-2-PhaP protein was immobilized on the surface of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx)-based nanoparticles. Loading of 240 μg (3.2 pMol) of B7-2-PhaP protein per mg nanoparticles was achieved. Immobilized B7-2-PhaP on PHBHHx nanoparticles induced T cell activation and proliferation in vitro. A PHA nanoparticle-based B7-2 costimulation molecule-presenting system was constructed. The PHA-based B7 presenting nanosystem provided costimulation signals to induce T cell activation and expansion in vitro. The B7-2-PhaP immobilized PHA nanosystem is a novel strategy for costimulation molecule presentation and may be used for costimulatory molecule complementary therapy.
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影响因子：
3.5
作者：
Bäckström BT;Brockelbank JA;Rehm BH
通讯作者：
Rehm BH
基于聚羟基脂肪酸酯颗粒结合蛋白 PhaP 与靶向细胞配体融合的特异性药物靶向系统
影响因子：
14
作者：
Yao, Yong-Chao;Zhan, Xiao-Yong;Chen, Guo-Qiang
通讯作者：
Chen, Guo-Qiang
影响因子：
2.1
作者：
Hang, XM;Lin, ZX;Chen, GQ
通讯作者：
Chen, GQ
影响因子：
6.4
作者：
Wang ZH;Ma P;Chen J;Zhang J;Chen CB;Chen GQ
通讯作者：
Chen GQ
影响因子：
3.6
作者：
Xiong, Yu-Cui;Yao, Yong-Chao;Chen, Guo-Qiang
通讯作者：
Chen, Guo-Qiang