Core Endophytic Bacteria and Their Roles in the Coralloid Roots of Cultivated Cycas revoluta (Cycadaceae).
Core Endophytic Bacteria and Their Roles in the Coralloid Roots of Cultivated Cycas revoluta (Cycadaceae).
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DOI：
10.3390/microorganisms11092364
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发表时间：
2023-09-21
期刊：
影响因子：
4.5
通讯作者：
Gong X
中科院分区：
文献类型：
--
作者：
Liu J;Xu H;Wang Z;Liu J;Gong X
作为裸子植物群，苏铁以其古老的起源和特化的珊瑚状根而闻名，可以作为探索宿主与相关微生物之间相互作用的理想系统。先前的研究表明，一些固氮蓝细菌对苏铁珊瑚状根内生微生物的组成有很大贡献。然而，宿主和环境在招募过程中塑造内生细菌组成的作用仍不清楚。在这里，我们确定了广泛栽培的苏铁（Cycas revoluta Thunb）珊瑚状根中内生细菌的多样性、组成和功能预测。利用二代测序技术，我们对中国11个地点的珊瑚根和土样土壤中的细菌多样性和群落结构进行了全面调查，旨在探索核心内生细菌的变化并预测其潜在功能。我们发现散装土壤中的微生物多样性高于珊瑚根中的微生物多样性。同时，不同地区内生细菌的多样性和组成没有显着差异，去除蓝藻后也有同样的结果。珊瑚状根中最主要的藻类为链单藻菌，其次是发菜菌，但这两种蓝藻并非所有样本都共有。红球菌属、Edaphobacter、Niastella、Nordella、SH-PL14和Virgisporangium被定义为珊瑚根中的核心微生物。功能预测分析表明，内生细菌主要参与植物对磷和金属离子的吸收以及抗病性。这些结果表明，珊瑚根中细菌的群落组成受到寄主和环境的共同影响，其中寄主更具决定性。尽管核心微生物所占比例很小，但它们的相互作用却很重要，并且可能有助于与宿主生存相关的功能。我们的研究有助于了解苏铁植物的微生物多样性和组成，并扩展了关于宿主与共生微生物之间关联的知识。
As a gymnosperm group, cycads are known for their ancient origin and specialized coralloid root, which can be used as an ideal system to explore the interaction between host and associated microorganisms. Previous studies have revealed that some nitrogen-fixing cyanobacteria contribute greatly to the composition of the endophytic microorganisms in cycad coralloid roots. However, the roles of host and environment in shaping the composition of endophytic bacteria during the recruitment process remain unclear. Here, we determined the diversity, composition, and function prediction of endophytic bacteria from the coralloid roots of a widely cultivated cycad, Cycas revoluta Thunb. Using next-generation sequencing techniques, we comprehensively investigated the diversity and community structure of the bacteria in coralloid roots and bulk soils sampled from 11 sites in China, aiming to explore the variations in core endophytic bacteria and to predict their potential functions. We found a higher microbe diversity in bulk soils than in coralloid roots. Meanwhile, there was no significant difference in the diversity and composition of endophytic bacteria across different localities, and the same result was found after removing cyanobacteria. Desmonostoc was the most dominant in coralloid roots, followed by Nostoc, yet these two cyanobacteria were not shared by all samples. Rhodococcus, Edaphobacter, Niastella, Nordella, SH-PL14, and Virgisporangium were defined as the core microorganisms in coralloid roots. A function prediction analysis revealed that endophytic bacteria majorly participated in the plant uptake of phosphorus and metal ions and in disease resistance. These results indicate that the community composition of the bacteria in coralloid roots is affected by both the host and environment, in which the host is more decisive. Despite the very small proportion of core microbes, their interactions are significant and likely contribute to functions related to host survival. Our study contributes to an understanding of microbial diversity and composition in cycads, and it expands the knowledge on the association between hosts and symbiotic microbes.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
11
作者：
Falcon, Luisa I.;Magallon, Susana;Castillo, Amanda
通讯作者：
Castillo, Amanda
DOI：
10.1073/pnas.2235808100
发表时间：
2003-11-11
影响因子：
11.1
作者：
Cox, PA;Banack, SA;Murch, SJ
通讯作者：
Murch, SJ
影响因子：
5.3
作者：
Zhou, Yongmei;Pang, Ziqin;Jia, Haifeng;Yuan, Zhaonian;Ming, Ray
通讯作者：
Ming, Ray
影响因子：
4.4
作者：
Compant, S;Reiter, B;Barka, EA
通讯作者：
Barka, EA