Responses of roots and rhizosphere of female papaya to the exogenous application of GA(3).
Responses of roots and rhizosphere of female papaya to the exogenous application of GA(3).
复制标题
DOI：
10.1186/s12870-022-04025-6
复制
发表时间：
2023-01-16
影响因子：
5.3
通讯作者：
Ming, Ray
中科院分区：
文献类型：
--
作者：
Zhou, Yongmei;Pang, Ziqin;Jia, Haifeng;Yuan, Zhaonian;Ming, Ray
外源GAs对根的发育有不确定的影响。本研究以雌性番木瓜为材料，通过研究GA 3处理和对照雌性番木瓜的根转录组、代谢谱和根际微生物群落，揭示了根系和根际对外源GA 3的响应。结果表明，外源GA 3处理促进了番木瓜雌株侧根的发育，使植株具有吸收水分和养分的物理优势。此外，在番木瓜茎尖喷施GA 3可能增加了生长素的水平，并通过CpPIN 1将其转运到根部，在根部生长素上调CpLBD 16并抑制CpBP，从而促进侧根的发生和发育。在番木瓜根系中，外源GA 3处理使相应的转运蛋白（CpTMT 3，CpNRT 1：2，CpPHT 1;4，CpINT 2，CpCOPT 2，CpABCB 11，CpNIP 4;1）表达上调，而使相应的排泄转运蛋白（CpNAXT 1）表达下调。此外，在GA 3处理的番木瓜根，CpALS 3和CpMYB 62下调，表明更强的非生物抗性铝毒和磷饥饿。另一方面，参与防御反应的BRs和JAs在GA 3处理的番木瓜根和根际中富集。这两种激素的上调可能导致根和根际病原菌如炭疽菌和轮枝菌的减少。GA 3处理的雌性木瓜增加了有益细菌物种的丰度，包括分枝杆菌、Mitsuaria和Actinophytocola，但减少了Candidatus和Bryobacter属的丰度，因为它需要更少的硝酸盐。总体而言，外源GA 3对雌木瓜根系和根际的生长发育和抗逆性有促进作用。GA 3处理雌性番木瓜可能通过上调CpLBD 16和下调CpBP的表达而促进侧根的形成和发育。GA 3处理的番木瓜根系表现出反馈控制的油菜素和茉莉酸信号在根的发育和防御。这些研究结果揭示了复杂的反应，生长激素治疗在番木瓜根和根际，并将导致调查其他植物激素对地下发展的番木瓜。在线版本包含补充材料，可通过10.1186/s12870-022-04025-6获得。
Exogenous GAs have an indeterminate effect on root development. Our current study used female papaya to reveal how the roots and rhizosphere respond to the exogenous application of GA3 by investigating the transcriptome profile in roots, metabolic profile and microbial community in both roots and rhizosphere of GA3-treated and control female papaya. The results demonstrated that exogenous GA3 treatment enhanced female papaya lateral root development, which gave plants physical advantages of water and nutrient uptake. In addition, it was likely that GA3 spraying in papaya shoot apices increased the level of auxin, which was transported to roots by CpPIN1, where auxin upregulated CpLBD16 and repressed CpBP to promote the lateral root initiation and development. In papaya roots, corresponding transporters (CpTMT3, CpNRT1:2, CpPHT1;4, CpINT2, CpCOPT2, CpABCB11, CpNIP4;1) were upregulated and excretion transporters were downregulated such as CpNAXT1 for water and nutrients uptake with exogenous GA3 application. Moreover, in GA3-treated papaya roots, CpALS3 and CpMYB62 were downregulated, indicating a stronger abiotic resistance to aluminum toxic and phosphate starvation. On the other hand, BRs and JAs, which involve in defense responses, were enriched in the roots and rhizosphere of GA3-treated papayas. The upregulation of the two hormones might result in the reduction of pathogens in roots and rhizosphere such as Colletotrichum and Verticillium. GA3-treated female papaya increased the abundance of beneficial bacteria species including Mycobacterium, Mitsuaria, and Actinophytocola, but decreased that of the genera Candidatus and Bryobacter for that it required less nitrate. Overall, the roots and rhizosphere of female papaya positively respond to exogenous application of GA3 to promote development and stress tolerance. Treatment of female papaya with GA3 might result in the promotion of lateral root formation and development by upregulating CpLBD16 and downregulating CpBP. GA3-treated papaya roots exhibited feedback control of brassinolide and jasmonate signaling in root development and defense. These findings revealed complex response to a growth hormone treatment in papaya roots and rhizosphere and will lead to investigations on the impact of other plant hormones on belowground development in papaya. The online version contains supplementary material available at 10.1186/s12870-022-04025-6.
登录
查看更多内容
影响因子：
9.1
作者：
Diao YZ;Zhang C;Liu F;Wang WZ;Liu L;Cai L;Liu XL
通讯作者：
Liu XL
影响因子：
6.9
作者：
Bose J;Babourina O;Shabala S;Rengel Z
通讯作者：
Rengel Z
外源 GA3 的应用改变了巨桉激素的形态、解剖和转录调控网络
影响因子：
2.9
作者：
Liu, Qian-Yu;Guo, Guang-Sheng;Fan, Chun-Jie
通讯作者：
Fan, Chun-Jie
影响因子：
4.2
作者：
Huang, Yali;Kuang, Zaoyuan;Cao, Lixiang
通讯作者：
Cao, Lixiang
DOI：
10.1016/j.bbrc.2020.08.025
发表时间：
2020-11-26
影响因子：
3.1
作者：
Li, Jianfu;Zhao, Chunyan;Zuo, Kaijing
通讯作者：
Zuo, Kaijing