Technical Considerations and Protocol Optimization for Neonatal Salivary Biomarker Discovery and Analysis.
Technical Considerations and Protocol Optimization for Neonatal Salivary Biomarker Discovery and Analysis.
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DOI：
10.3389/fped.2020.618553
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发表时间：
2020
影响因子：
2.6
通讯作者：
Maron JL
中科院分区：
文献类型：
--
作者：
Yen E;Kaneko-Tarui T;Maron JL
非侵入性技术监测和诊断新生儿，特别是早产儿，是新生儿医学长期追求的目标。近年来，技术的进步，加上检测灵敏度的提高，使得从单一样品来源同时进行多个生物标志物的高通量分析成为可能。多重转录和蛋白质组平台，加上更全面的分析，如RNAseq，允许以前所未有的方式询问正在进行的生理学和病理学。在脆弱的新生儿群体中，唾液是一种理想的生物流体，可以用来连续评估临床状况，并比更具侵入性的血液样本具有许多优势。重要的是，唾液样本可以在新兴的蛋白质组和转录平台上进行分析，即使数量有限。然而，生物标记物靶标通常在人类唾液中被降解，作为一种包含人类和微生物靶标的混合源生物流体，唾液对研究者提出了独特的挑战。在这里，我们提供了我们实验室开发的技术考虑和方案优化的见解，以提高重复性和可靠性来量化和发现新生儿唾液生物标记物。我们将详细介绍从我们实验室多年的新生儿唾液实验中学到的见解，从唾液收集技术到处理再到下游分析，强调方法的一致性和对新生儿唾液生物标记物分析的潜在好处和局限性的全球理解的必要性。重要的是，我们将强调在这一人群中进行强有力和严格的研究的必要性，以便为该领域提供标准化的方法和工作流程，以成功地影响新生儿护理。
Non-invasive techniques to monitor and diagnose neonates, particularly those born prematurely, are a long-sought out goal of Newborn Medicine. In recent years, technical advances, combined with increased assay sensitivity, have permitted the high-throughput analysis of multiple biomarkers simultaneously from a single sample source. Multiplexed transcriptomic and proteomic platforms, along with more comprehensive assays such as RNASeq, allow for interrogation of ongoing physiology and pathology in unprecedented ways. In the fragile neonatal population, saliva is an ideal biofluid to assess clinical status serially and offers many advantages over more invasively obtained blood samples. Importantly, saliva samples are amenable to analysis on emerging proteomic and transcriptomic platforms, even at quantitatively limited volumes. However, biomarker targets are often degraded in human saliva, and as a mixed source biofluid containing both human and microbial targets, saliva presents unique challenges for the investigator. Here, we provide insight into technical considerations and protocol optimizations developed in our laboratory to quantify and discover neonatal salivary biomarkers with improved reproducibility and reliability. We will detail insights learned from years of experimentation on neonatal saliva within our laboratory ranging from salivary collection techniques to processing to downstream analyses, highlighting the need for consistency in approach and a global understanding of both the potential benefits and limitations of neonatal salivary biomarker analyses. Importantly, we will highlight the need for robust and stringent research in this population to provide the field with standardized approaches and workflows to impact neonatal care successfully.
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影响因子：
3.7
作者：
Hicks, Steven D.;Rajan, Alexander T.;Middleton, Frank A.
通讯作者：
Middleton, Frank A.
影响因子：
5.4
作者：
Maron, Jill L.
通讯作者：
Maron, Jill L.
影响因子：
5.1
作者：
Maron, Jill L.;Hwang, Jooyeon S.;Alterovitz, Gil
通讯作者：
Alterovitz, Gil
影响因子：
3.7
作者：
Zhao S;Fung-Leung WP;Bittner A;Ngo K;Liu X
通讯作者：
Liu X
DOI：
10.3109/14767058.2012.717126
发表时间：
2012-12
期刊：
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians
影响因子：
--
作者：
Maron JL;Dietz JA;Parkin C;Johnson KL;Bianchi DW
通讯作者：
Bianchi DW