Characterization of two homologues of ChaB in Spodoptera litura multicapsid nucleopolyhedrovirus.
Characterization of two homologues of ChaB in Spodoptera litura multicapsid nucleopolyhedrovirus.
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斜纹夜蛾多衣壳核多角体病毒中 ChaB 的两个同源物的表征。
作者：
ChaB是大肠杆菌（Escherichia coli，E.大肠杆菌），及其同源物构成了已知存在于细菌、古细菌和杆状病毒中的多基因家族。与E.大肠杆菌ChaB、杆状病毒ChaB蛋白缺少一个能与Ca ~（2+）和Mg ~（2+）结合的带电环。杆状病毒斜纹夜蛾核多角体病毒（Spodoptera litura multicapsid nucleopolyhedrovirus，SpltMNPV）含有Cha B的两个同源物，开放阅读框（ORF）52（S152）和53（S153）。逆转录PCR和5′端Race分析表明，两种基因的转录均在感染后12 h开始，起始于晚期共有（A/T）TAAG基序。免疫印迹分析表明，在感染的S.而Sl 53表达为16 kDa的蛋白。生化分级分析表明，23 kDa形式的S152分布在细胞质和细胞核中，而26 kDa形式的S152和S153蛋白只存在于细胞核中。进一步的分析表明，这些蛋白质与闭塞衍生病毒的核衣壳相关。使用组蛋白提取方案，在组蛋白H1级分中检测到S153和26 kDa形式的S152蛋白。此外，柱层析分析表明，S152和S153蛋白可以与核酸相互作用。推测SpltMNPV ChaB可能具有DNA结合蛋白的功能。
ChaB, a putative regulator of ChaA in Escherichia coli (E. coli), and its homologues constitute a multigene family known to occur among bacteria, archaeabacteria and baculoviruses. Distinguished from E. coli ChaB, baculoviruses ChaB proteins lack a charged loop that can bind to Ca2+and Mg2+. The baculovirus Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) contains two homologues of ChaB, open reading frames (ORFs) 52 (Sl52) and 53 (Sl53). Reverse transcription-PCR and 5′ Race analyses indicated that transcription of both SpltMNPV chaB genes occurs by 12 h postinfection and is initiated at a late consensus (A/T)TAAG motif. Immunoblot analysis showed that Sl52 was expressed as a doublet of 23 and 26 kDa in infected S. litura cells, while Sl53 was expressed as a 16 kDa protein. Biochemical fractionation analysis indicated that the 23 kDa form of Sl52 was distributed in both cytoplasm and nucleus of infected cells, whereas the 26 kDa form of Sl52 and Sl53 proteins were only present in nucleus. Further analysis revealed that these proteins are associated with the nucleocapsid of occlusion-derived virus. Using a histone extraction protocol, the Sl53 and 26 kDa form of Sl52 proteins were both detected in the histone H1 fraction. Additionally, column chromatography analysis showed that the Sl52 and Sl53 proteins could interact with nucleic acids. It was proposed that SpltMNPV ChaB might function as DNA binding proteins.
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DOI：
10.1016/s0005-2728(97)00102-3
发表时间：
1998-03
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Tatsuya Sakuma;Naomi Yamada;H. Saito;T. Kakegawa;Hiroshi Kobayashi
通讯作者：
Tatsuya Sakuma;Naomi Yamada;H. Saito;T. Kakegawa;Hiroshi Kobayashi
DOI：
10.1016/s0005-2728(02)00345-6
发表时间：
2002-12
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
T. Shijuku;T. Yamashino;Hyuma Ohashi;H. Saito;T. Kakegawa;M. Ohta;Hiroshi Kobayashi
通讯作者：
T. Shijuku;T. Yamashino;Hyuma Ohashi;H. Saito;T. Kakegawa;M. Ohta;Hiroshi Kobayashi
影响因子：
3.7
作者：
JARVIS, DL;BOHLMEYER, DA;GARCIA, A
通讯作者：
GARCIA, A
影响因子：
13.8
作者：
Dandekar, T;Snel, B;Bork, P
通讯作者：
Bork, P
影响因子：
1.6
作者：
Zhaofei Li;Cui Li;Lijing Pan;Mei Yu;Kai Yang;Y. Pang
通讯作者：
Zhaofei Li;Cui Li;Lijing Pan;Mei Yu;Kai Yang;Y. Pang