Insights into the evolution of Archaea and eukaryotic protein modifier systems revealed by the genome of a novel archaeal group.
Insights into the evolution of Archaea and eukaryotic protein modifier systems revealed by the genome of a novel archaeal group.
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DOI：
10.1093/nar/gkq1228
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发表时间：
2011-04
影响因子：
14.9
通讯作者：
Takami H
中科院分区：
文献类型：
--
作者：
Nunoura T;Takaki Y;Kakuta J;Nishi S;Sugahara J;Kazama H;Chee GJ;Hattori M;Kanai A;Atomi H;Takai K;Takami H
古生界在历史上被分为两个门，Crenarchaeota和Euryarchaeota。虽然被认为是Crenarchaeota的小亚基rRNA基因组学的基础上的成员，环境基因组学和努力培养最近揭示了两个新的门/司在古菌;'Thaumarchaeota'和'Korarchaeota'。在这里，我们显示的基因组序列的Caldiarchaeum subjuveneum '，代表一个未开垦的crenarchaeotic组。从先前从地下金矿地热水流处的微生物垫制备的宏基因组文库重建复合基因组。发现该基因组与已知的门/部门，Crenarchaeota（hyperthermophiles），Euryarchaeota，Thaumarchaeota和Korarchaeota的基因组明显不同。独特的特征表明，这个crenarchaeotic组可以被认为是一个新的古门/司。此外，C. subacryeum具有由Ub、E1、E2和小Zn RING指家族蛋白组成的泛素样蛋白修饰剂系统，其具有对真核系统蛋白特异的结构基序，该系统与最近在Haloferax和分枝杆菌中鉴定的原核生物型系统明显不同。这种真核生物型系统的存在是前所未有的原核生物，并表明真核生物蛋白质修饰剂系统的原型是存在于的。
The domain Archaea has historically been divided into two phyla, the Crenarchaeota and Euryarchaeota. Although regarded as members of the Crenarchaeota based on small subunit rRNA phylogeny, environmental genomics and efforts for cultivation have recently revealed two novel phyla/divisions in the Archaea; the ‘Thaumarchaeota’ and ‘Korarchaeota’. Here, we show the genome sequence of Candidatus ‘Caldiarchaeum subterraneum’ that represents an uncultivated crenarchaeotic group. A composite genome was reconstructed from a metagenomic library previously prepared from a microbial mat at a geothermal water stream of a sub-surface gold mine. The genome was found to be clearly distinct from those of the known phyla/divisions, Crenarchaeota (hyperthermophiles), Euryarchaeota, Thaumarchaeota and Korarchaeota. The unique traits suggest that this crenarchaeotic group can be considered as a novel archaeal phylum/division. Moreover, C. subterraneum harbors an ubiquitin-like protein modifier system consisting of Ub, E1, E2 and small Zn RING finger family protein with structural motifs specific to eukaryotic system proteins, a system clearly distinct from the prokaryote-type system recently identified in Haloferax and Mycobacterium. The presence of such a eukaryote-type system is unprecedented in prokaryotes, and indicates that a prototype of the eukaryotic protein modifier system is present in the Archaea.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
2.9
作者：
Burroughs, A. Maxwell;Iyer, Lakshminarayan M.;Aravind, L.
通讯作者：
Aravind, L.
影响因子：
14.9
作者：
Grissa, Ibtissem;Vergnaud, Gilles;Pourcel, Christine
通讯作者：
Pourcel, Christine
DOI：
10.1073/pnas.0801980105
发表时间：
2008-06-10
影响因子：
11.1
作者：
Elkins, James G.;Podar, Mircea;Stetter, Karl O.
通讯作者：
Stetter, Karl O.
影响因子：
56.9
作者：
FLEISCHMANN, RD;ADAMS, MD;VENTER, JC
通讯作者：
VENTER, JC