Polymorphic karyotypes in related Acremonium strains

Polymorphic karyotypes in related Acremonium strains
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相关枝顶孢属菌株的多态性核型

DOI:
10.1007/bf00326285
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发表时间:
1991
期刊:
影响因子:
2.5
通讯作者:
U. Kück
U. Kück
中科院分区:
生物学3区
文献类型:
--
作者:
M. Walz;U. Kück

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摘要对6株相关的顶孢菌进行限制性片段长度多态性(RFLP)分析。在限制性片段模式上,所有菌株之间没有明显区别,但与A. strictum、A. flavum和Cephalosporium polyvaleurum有明显差异。利用脉冲场凝胶电泳技术,我们得到了大多数阿克蒙菌不同的染色体模式。值得注意的是,三种相关的黄孢杆菌菌株的模式不同,它们的头孢菌素C生物合成率也不同。rDNA基因在不同染色体上的定位证实了电泳核型。我们的数据表明,染色体易位在工业菌株可能负责增加β-内酰胺合成。
SummaryA restriction fragment length polymorphism (RFLP) analysis was performed on six related Acremonium strains. With respect to the restriction fragment pattern, all strains of A. chrysogenum were indistinguishable from each other but showed distinctive differences from those of A. strictum, A. flavum and Cephalosporium polyvaleurum. Using pulsed-field gel electrophoresis, we obtained different chromosome patterns from most of the Acremonium strains. Remarkably, the pattern varies in three related A. chrysogenum strains which also differ in their rate of cephalosporin C biosynthesis. The electrophoretic karyotyping was confirmed by the location of rDNA genes on separate chromosomes. Our data indicate that chromosome translocations in industrial strains may be responsible for increased β-lactam synthesis.
通过轮廓钳制均匀电场分辨率大于 5 兆碱基的 DNA 分子。
DOI: 10.1093/nar/15.19.7865
发表时间: 1987
影响因子: 14.9
作者:
Vollrath,D;Davis,RW
通讯作者: Davis,RW
DOI: 10.1073/pnas.86.16.6260
发表时间: 1989
影响因子: 11.1
作者:
Brody,H;Carbon,J
通讯作者: Carbon,J