Yeast artificial chromosome cloning of the β-catenin locus on human chromosome 3p21–22
Yeast artificial chromosome cloning of the β-catenin locus on human chromosome 3p21–22
复制标题
人类染色体 3p21-22 上 β-连环蛋白基因座的酵母人工染色体克隆
作者：
A. Bailey;A. Norris;J. Leek;P. Clissold;I. Carr;D. Ogilvie;J. Morrison;D. Meredith;A. Markham
摘要β-连环蛋白是上皮细胞间粘附连接的重要组成部分。由于其与apc基因产物相互作用的研究结果，它与结直肠癌的发展有关。α-Catenin、β-catenin、E-cadherin和APC似乎介导了上皮细胞的接触抑制。作为β-catenin基因组织研究的一部分，我们分离了酵母人工染色体（YACs）来表征其内含子/外显子结构。体细胞杂交dna的YAC荧光原位杂交分析和聚合酶链反应分析表明，β-catenin位于3p21-22区域，这是小细胞肺癌（SCLC）和其他疾病中缺失的肿瘤抑制基因的位置。β-Catenin YACs将为杂合性缺失研究提供有用的微卫星标记来源，以评估β-Catenin缺失在SCLC中的重要性。
Abstractβ-Catenin has emerged as an important component of the adherens junctions between epithelial cells. As a result of studies of its interaction with theAPC gene product, it has been implicated in the development of colorectal cancer. α-Catenin, β-catenin, E-cadherin and APC appear to mediate contact inhibition in epithelia. As part of the study of the organization of the β-catenin gene, we have isolated yeast artificial chromosomes (YACs) to characterize its intron/exon structure. YAC fluorescencein situ hybridization analysis and polymerase chain reaction analysis of somatic cell hybrid DNAs show that β-catenin maps in the 3p21–22 region, the location of tumour-suppressor genes deleted in small-cell lung cancer (SCLC) and other disorders. β-Catenin YACs will provide a source of microsatellite markers useful in loss of heterozygosity studies to assess the importance of β-catenin deletions in SCLC.
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原发性透明细胞肾癌染色体 3p12 和 3p14.2 区域的常见缺失区域。
影响因子：
11.2
作者：
Lubinski,J;Hadaczek,P;Podolski,J;Toloczko,A;Sikorski,A;McCue,P;Druck,T;Huebner,K
通讯作者：
Huebner,K
影响因子：
11.2
作者：
A. Merlo;M. Mabry;E. Gabrielson;R. Vollmer;S. Baylin;D. Sidransky
通讯作者：
A. Merlo;M. Mabry;E. Gabrielson;R. Vollmer;S. Baylin;D. Sidransky
影响因子：
56.9
作者：
PAPADOPOULOS, N;NICOLAIDES, NC;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
影响因子：
56.9
作者：
SU, LK;VOGELSTEIN, B;KINZLER, KW
通讯作者：
KINZLER, KW