Not All Side Population Cells Contain Cancer Stem-Like Cells in Human Gastric Cancer Cell Lines
Not All Side Population Cells Contain Cancer Stem-Like Cells in Human Gastric Cancer Cell Lines
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人胃癌细胞系中并非所有侧群细胞都含有癌症干样细胞
DOI：
10.1007/s10620-012-2330-1
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发表时间：
2012
影响因子：
3.1
通讯作者：
Zikuan Guo
中科院分区：
文献类型：
--
作者：
Zhou Song;Hua Wang;Qinfang Li;Zikuan Guo
SP细胞在肿瘤的发生和复发中起着重要的作用。SP细胞在多种细胞系和组织中均有表达，包括胃癌细胞系。然而，在胃癌细胞系中是否所有SP细胞都含有癌干细胞样细胞？材料与方法选用Hoechst 33342标记的MKN-45和BGC-823细胞，获得SP细胞，并在体内外对SP细胞的肿瘤干细胞样特性进行了研究。采用实时荧光定量RT-PCR方法检测SP和MP细胞中OCT-4、SOX-2、NANOG、CD 44和ATP结合盒转运体基因ABCG-2等5个干细胞相关基因的表达谱。Western blot检测SP和MP细胞蛋白表达的差异。MKN-45来源的SP细胞接种非肥胖型糖尿病/严重联合免疫缺陷（NOD/SCID）小鼠后，其致瘤性高于MP细胞，而BGC-823来源的SP细胞则与MP细胞致瘤性相同。BGC-823来源的SP细胞不具有肿瘤干细胞特性，证明胃癌细胞系中并非所有SP细胞都含有肿瘤干细胞样细胞。
IntroductionSide population (SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer. Many kinds of cell lines and tissue have demonstrated presence of SP cells including different gastric cancer cell lines. However, is that true all SP cells contain cancer stem-like cells in gastric cancer cell lines?Materials and methodsMKN-45 and BGC-823 cells labeled with Hoechst 33342 were chosen to obtain SP cells, then characterized the cancer stem-like properties of SP cells both in vitro and in vivo. Five stemness–related genes expression profiles, including OCT-4, SOX-2, NANOG, CD44 and ATP-binding cassette transporters gene ABCG-2, were tested in SP and MP cells using quantitative real-time RT-PCR. Western blot was chosen to show the difference of protein expression between SP and MP cells. When inoculated into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice, SP cells from MKN-45 showed higher tumorigenesis tendency than MP cells, but SP cells from BGC-823 showed same tumorgenesis tendency as MP cells.ConclusionSP cells from MKN-45 possess cancer stem cell properties and proved that they were gastric cancer stem-like cells. SP cells from BGC-823 didn’t possess cancer stem cell properties and proved that not all SP cells contain cancer stem-like cells in gastric cancer cell lines.
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作者：
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影响因子：
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