Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples
Systematic Analysis of Intestinal MicroRNAs Expression in HCC: Identification of Suitable Reference Genes in Fecal Samples
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HCC 中肠道 MicroRNA 表达的系统分析：粪便样本中合适参考基因的鉴定
DOI：
10.3389/fgene.2019.00687
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发表时间：
2019-08
影响因子：
3.7
通讯作者：
Yunlong Bai
中科院分区：
文献类型：
--
作者：
Hui Wang;Yuan Lv;Cao Wang;Dongjing Leng;Yan Yan;Moyondafoluwa Blessing Fasae;Syeda Madiha Zahra;Yanan Jiang;Zhiguo Wang;Baofeng Yang;Yunlong Bai
肝细胞癌（HCC）是一种非常致命的恶性肿瘤。在人类粪便样本中可以检测到的肠道microRNA可能参与HCC的病理过程。因此，在粪便样本中筛选功能性肠道microRNA并研究其在HCC分子进展中的潜在作用是必要的。实时定量PCR（qRT-PCR）已广泛应用于microRNA表达研究。然而，很少有基因被报道为粪便样品中肠道microRNA的参考基因。为了获得更准确分析的肠道microRNA表达，我们首先使用三个软件包（GeNorm、Normandy和Bestkeeper）在qRT-PCR期间搜索用于肠道microRNA表达标准化的可靠参考基因。接下来我们通过定量RT-PCR或miRtarBase筛选并预测了对照组和HCC小鼠肠道差异microRNA的靶基因。最后，我们还使用大卫生物信息学资源数据库分析了基因本体论（GO）术语和京都基因和基因组百科全书（KEGG）途径富集的mRNA靶标。本研究成功筛选了相对合适的参考基因，发现肠道差异microRNA在肝癌的发生发展中起重要作用。本研究确定的最佳参考基因可为合理选择合适的参考基因提供理论依据。此外，检测肠道microRNA的表达可能成为肝癌诊断和治疗的一个有前途的治疗靶点。
Hepatocellular carcinoma (HCC) is an extremely fatal malignancy. Intestinal microRNAs, which can be detected in fecal samples in humans may be involved in the pathological process of HCC. Therefore, screening for functional intestinal microRNAs in fecal samples and investigating their potential roles in the molecular progression of HCC are necessary. Quantitative real-time PCR (qRT-PCR) has been widely used in microRNA expression studies. However, few genes have been reported as reference genes for intestinal microRNAs in fecal samples. In order to obtain a more accurately analyzed intestinal microRNAs expression, we first searched for reliable reference genes for intestinal microRNAs expression normalization during qRT-PCR, using three software packages (GeNorm, NormFinder, and Bestkeeper). Next we screened and predicted the target genes of the differentially intestinal microRNAs of control and HCC mice through quantitative RT-PCR or miRtarBase. Finally, we also analyzed the mRNA targets for enrichment of Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways using the DAVID Bioinformatic Resources database. This study has successfully screened relatively suitable reference genes and we have discovered that the differential intestinal microRNAs play significant roles in the development of HCC. The top reference genes identified in this study could provide a theoretical foundation for the reasonable selection of a suitable reference gene. Furthermore, the detection of intestinal microRNAs expression may serve as a promising therapeutic target for the diagnosis and treatment of HCC.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
30.3
作者：
Liu S;da Cunha AP;Rezende RM;Cialic R;Wei Z;Bry L;Comstock LE;Gandhi R;Weiner HL
通讯作者：
Weiner HL
对蜜蜂幼虫的孵化刺的调查，包括Apis Mellifera（膜翅目：apoidea）。
DOI：
10.1093/jisesa/iex060
发表时间：
2017-07-01
期刊：
Journal of insect science (Online)
影响因子：
--
作者：
Rozen JG Jr;Shepard Smith C;Cane JH
通讯作者：
Cane JH
DOI：
10.1016/j.clinre.2014.04.008
发表时间：
2014-12-01
影响因子：
2.7
作者：
Li, Xiaofei;Yang, Wenjun;Lou, Lianqing
通讯作者：
Lou, Lianqing
影响因子：
11.5
作者：
Sahin, F;Kannangai, R;Torbenson, M
通讯作者：
Torbenson, M