Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor.
Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor.
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鉴定使用BAT ACE2受体的新型谱系蝙蝠SARS相关的冠状病毒。
DOI：
10.1080/22221751.2021.1956373
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发表时间：
2021-12
影响因子：
13.2
通讯作者：
Shi ZL
中科院分区：
文献类型：
--
作者：
Guo H;Hu B;Si HR;Zhu Y;Zhang W;Li B;Li A;Geng R;Lin HF;Yang XL;Zhou P;Shi ZL
严重呼吸道疾病冠状病毒2(SARS-CoV-2)已成为本世纪最具破坏性的新冠肺炎疾病。SARS-CoV-2尚未解决的科学问题之一是这种病毒的动物来源。蝙蝠和穿山甲被认为是最有可能携带高度相似的SARS-CoV-2相关病毒(SARSR-CoV-2)的宿主。这项研究从2015年我们发现RaTG13的同一地点的蝙蝠身上发现了一种新的SARSR-CoV谱系，包括RaTG15和其他七种病毒。虽然RaTG15及相关病毒与SARS-CoV-2在保守的ORF1b区有97.2%的氨基酸序列同源性，但在基因组水平上与所有已知的SARSR-CoV的核苷酸同源性不到77.6%，从而形成了一个独特的系统发育树。我们发现RaTG15受体结合域(RBD)可以与马来亚穿山甲的ACE2结合，并将其用作进入受体，但人类的ACE2除外。然而，它包含一个短的缺失，并具有不同的负责ACE2结合的关键残基。此外，我们还发现，在已发现的蝙蝠SARSR-CoV-2谱系中，没有一种病毒像穿山甲衍生的SARSR-CoV-2或SARSR-CoV-1谱系中的某些病毒那样有效地利用人类ACE2。因此，需要对蝙蝠进行进一步的系统和纵向研究，以防止未来由SARSR-CoV引起的溢出事件或更好地了解SARS-CoV-2的起源。
Severe respiratory disease coronavirus-2 (SARS-CoV-2) has been the most devastating disease COVID-19 in the century. One of the unsolved scientific questions of SARS-CoV-2 is the animal origin of this virus. Bats and pangolins are recognized as the most probable reservoir hosts that harbour highly similar SARS-CoV-2 related viruses (SARSr-CoV-2). This study identified a novel lineage of SARSr-CoVs, including RaTG15 and seven other viruses, from bats at the same location where we found RaTG13 in 2015. Although RaTG15 and the related viruses share 97.2% amino acid sequence identities with SARS-CoV-2 in the conserved ORF1b region, it only shows less than 77.6% nucleotide identity to all known SARSr-CoVs at the genome level, thus forming a distinct lineage in the Sarbecovirus phylogenetic tree. We found that the RaTG15 receptor-binding domain (RBD) can bind to ACE2 from Rhinolophus affinis, Malayan pangolin, and use it as an entry receptor, except for ACE2 from humans. However, it contains a short deletion and has different key residues responsible for ACE2 binding. In addition, we showed that none of the known viruses in bat SARSr-CoV-2 lineage discovered uses human ACE2 as efficiently as the pangolin-derived SARSr-CoV-2 or some viruses in the SARSr-CoV-1 lineage. Therefore, further systematic and longitudinal studies in bats are needed to prevent future spillover events caused by SARSr-CoVs or to understand the origin of SARS-CoV-2 better.
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影响因子：
82.9
作者：
Menachery VD;Yount BL Jr;Debbink K;Agnihothram S;Gralinski LE;Plante JA;Graham RL;Scobey T;Ge XY;Donaldson EF;Randell SH;Lanzavecchia A;Marasco WA;Shi ZL;Baric RS
通讯作者：
Baric RS
影响因子：
5.4
作者：
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通讯作者：
Ritter DG
影响因子：
5.4
作者：
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通讯作者：
Shi, Zheng-Li
影响因子：
16.6
作者：
Wacharapluesadee S;Tan CW;Maneeorn P;Duengkae P;Zhu F;Joyjinda Y;Kaewpom T;Chia WN;Ampoot W;Lim BL;Worachotsueptrakun K;Chen VC;Sirichan N;Ruchisrisarod C;Rodpan A;Noradechanon K;Phaichana T;Jantarat N;Thongnumchaima B;Tu C;Crameri G;Stokes MM;Hemachudha T;Wang LF
通讯作者：
Wang LF
病毒与宿主之间的进化军备竞赛驱动蝙蝠严重急性呼吸系统综合症相关冠状病毒刺突基因的遗传多样性
影响因子：
5.4
作者：
Guo H;Hu BJ;Yang XL;Zeng LP;Li B;Ouyang S;Shi ZL
通讯作者：
Shi ZL