Evidence for SARS-CoV-2 related coronaviruses circulating in bats and pangolins in Southeast Asia.
Evidence for SARS-CoV-2 related coronaviruses circulating in bats and pangolins in Southeast Asia.
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DOI：
10.1038/s41467-021-21240-1
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发表时间：
2021-02-09
影响因子：
16.6
通讯作者：
Wang LF
中科院分区：
文献类型：
--
作者：
Wacharapluesadee S;Tan CW;Maneeorn P;Duengkae P;Zhu F;Joyjinda Y;Kaewpom T;Chia WN;Ampoot W;Lim BL;Worachotsueptrakun K;Chen VC;Sirichan N;Ruchisrisarod C;Rodpan A;Noradechanon K;Phaichana T;Jantarat N;Thongnumchaima B;Tu C;Crameri G;Stokes MM;Hemachudha T;Wang LF
COVID-19 大流行的许多问题尚未得到解答，其中包括 SARS-CoV-2 的起源以及中间动物宿主在早期动物向人类传播中的潜在作用。在中国发现的RaTG13蝙蝠冠状病毒表明蝙蝠起源的可能性很高。在此，我们报告了在东南亚蝙蝠中活跃传播的 SARS-CoV-2 相关冠状病毒 (SC2r-CoV) 的分子和血清学证据。从泰国洞穴中的五只独立蝙蝠（犀牛蝠）获得全基因组序列，产生单一分离株（名为 RacCS203），该分离株与中国云南马来菊头蝠中发现的 RmYN02 分离株最相关。在同一群体的蝙蝠和泰国南部野生动物检查站的穿山甲中也检测到了 SARS-CoV-2 中和抗体。尽管 RacCS203 或 RmYN02 的 RBD 未能结合 ACE2，但针对 RmYN02 受体结合域 (RBD) 的抗血清能够交叉中和 SARS-CoV-2。尽管病毒的起源仍未解决，但我们的研究将遗传多样性 SC2r-CoV 的地理分布范围从日本和中国扩展到泰国，覆盖范围超过 4800 公里。迫切需要跨境监测来寻找 SARS-CoV-2 的直接祖病毒。有人提出 SARS-CoV-2 源自蝙蝠。在这里，作者通过对泰国野生犀牛蝙蝠进行采样，鉴定出一种与 SARS-CoV-2 相关的冠状病毒 (SC2r-CoV)，命名为 RacCS203，与 SARS-CoV-2 具有 91.5% 的基因组相似性，并表明从蝙蝠和马来穿山甲获得的血清可以中和 SARS-CoV-2。
Among the many questions unanswered for the COVID-19 pandemic are the origin of SARS-CoV-2 and the potential role of intermediate animal host(s) in the early animal-to-human transmission. The discovery of RaTG13 bat coronavirus in China suggested a high probability of a bat origin. Here we report molecular and serological evidence of SARS-CoV-2 related coronaviruses (SC2r-CoVs) actively circulating in bats in Southeast Asia. Whole genome sequences were obtained from five independent bats (Rhinolophus acuminatus) in a Thai cave yielding a single isolate (named RacCS203) which is most related to the RmYN02 isolate found in Rhinolophus malayanus in Yunnan, China. SARS-CoV-2 neutralizing antibodies were also detected in bats of the same colony and in a pangolin at a wildlife checkpoint in Southern Thailand. Antisera raised against the receptor binding domain (RBD) of RmYN02 was able to cross-neutralize SARS-CoV-2 despite the fact that the RBD of RacCS203 or RmYN02 failed to bind ACE2. Although the origin of the virus remains unresolved, our study extended the geographic distribution of genetically diverse SC2r-CoVs from Japan and China to Thailand over a 4800-km range. Cross-border surveillance is urgently needed to find the immediate progenitor virus of SARS-CoV-2. A bat origin for SARS-CoV-2 has been proposed. Here, by sampling wild Rhinolophus acuminatus bats from Thailand, the authors identified a SARS-CoV-2-related coronavirus (SC2r-CoV), designated as RacCS203, with 91.5% genome similarity to SARS-CoV-2, and show that sera obtained from bats and Malayan pangolin neutralize SARS-CoV-2.
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