Autophagy-related protein MoAtg14 is involved in differentiation, development and pathogenicity in the rice blast fungus Magnaporthe oryzae.
Autophagy-related protein MoAtg14 is involved in differentiation, development and pathogenicity in the rice blast fungus Magnaporthe oryzae.
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自噬相关蛋白 MoAtg14 参与稻瘟病菌 Magnaporthe oryzae 的分化、发育和致病性
DOI：
10.1038/srep40018
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发表时间：
2017-01-09
影响因子：
4.6
通讯作者：
Lin FC
中科院分区：
文献类型：
--
作者：
Liu XH;Zhao YH;Zhu XM;Zeng XQ;Huang LY;Dong B;Su ZZ;Wang Y;Lu JP;Lin FC
自噬是真核细胞内主要的细胞内降解系统，细胞质物质通过自噬进入空泡/溶酶体并在空泡/溶酶体中降解。MoAtg 14在M. Atg 14是一种迄今尚未鉴定的蛋白质，是酵母Atg 14和哺乳动物BARKOR的高度分化的同源物。MoATG 14缺失突变体在菌落中心表现出塌陷，分生孢子形成不良，毒力完全丧失。值得注意的是，Δ Moatg 14突变体表现出延迟的糖原分解，较少的脂质体，降低附着胞的膨压和受损的分生孢子自噬细胞死亡。自噬过程在Δ Moatg 14突变体中被阻断，标志蛋白GFP-MoAtg 8的自噬降解被中断。GFP-MoAtg 14与mCherry-MoAtg 8共定位于气生菌丝中。此外，一个保守的卷曲螺旋结构域的MoAtg 14蛋白的N-末端区域，该结构域可以介导MoAtg 14和MoAtg 6之间的相互作用预测。MoAtg 14蛋白的卷曲螺旋结构域对于其在自噬和致病性中的功能是必不可少的。
Autophagy is the major intracellular degradation system by which cytoplasmic materials are delivered to and degraded in the vacuole/lysosome in eukaryotic cells. MoAtg14 in M. oryzae, a hitherto uncharacterized protein, is the highly divergent homolog of the yeast Atg14 and the mammal BARKOR. The MoATG14 deletion mutant exhibited collapse in the center of the colonies, poor conidiation and a complete loss of virulence. Significantly, the ΔMoatg14 mutant showed delayed breakdown of glycogen, less lipid bodies, reduced turgor pressure in the appressorium and impaired conidial autophagic cell death. The autophagic process was blocked in the ΔMoatg14 mutant, and the autophagic degradation of the marker protein GFP-MoAtg8 was interrupted. GFP-MoAtg14 co-localized with mCherry-MoAtg8 in the aerial hypha. In addition, a conserved coiled-coil domain was predicted in the N-terminal region of the MoAtg14 protein, a domain which could mediate the interaction between MoAtg14 and MoAtg6. The coiled-coil domain of the MoAtg14 protein is essential for its function in autophagy and pathogenicity.
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发表时间：
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期刊：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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