Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line.
Establishment and characterization of pygmy killer whale (Feresa attenuata) dermal fibroblast cell line.
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侏儒虎鲸 (Feresa attenuata) 真皮成纤维细胞系的建立和表征
DOI：
10.1371/journal.pone.0195128
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Wenhua L
中科院分区：
文献类型：
--
作者：
Yajing S;Rajput IR;Ying H;Fei Y;Sanganyado E;Ping L;Jingzhen W;Wenhua L
侏儒虎鲸（学名：Feresa attenuata）是一种热带和亚热带海洋哺乳动物，常见于大西洋、印度洋和太平洋。由于PKW生活在近海保护区，他们很少在岸上看到。因此，PKW是最不了解的海洋物种之一。皮肤组织主要来自发生在中国广东汕头沿着的搁浅事件。立即处理取样的组织并将其附着在胶原包被的6孔组织培养板上。向培养板中加入完全培养基（DMEM和Ham's F12、胎牛血清、抗生素和必需氨基酸）。原代培养的PKW-LWH细胞经波形蛋白和核型分析证实为成纤维细胞，显示42条常染色体和两条性染色体X和Y。用编码SV 40大T抗原的质粒转染PKW-LWH细胞后，分离并扩增转染的细胞。采用RT-PCR、蛋白质印迹、免疫荧光分析和SV 40大T抗原稳定性得到证实。成纤维细胞PKW-LWHT的细胞增殖速度比原代细胞PKW-LWH快，倍增时间分别为68.9h和14.4h。在这项研究中，我们建立了PKW真皮成纤维细胞系的第一次，提供了一个独特的机会，在体外研究的影响，环境污染物和病原体，可以确定在PKW和/或鲸类动物。
The pygmy killer whale (Feresa attenuata) (PKW) is a tropical and subtropical marine mammal commonly found in the Atlantic, Indian and Pacific oceans. Since the PKWs live in offshore protected territories, they are rarely seen onshore. Hence, PKW are one of the most poorly understood oceanic species of odontocetes. The dermal tissue comes primarily from stranding events that occur along the coast of the Shantou, Guangdong, China. The sampled tissues were immediately processed and attached on collagen-coated 6-well tissue culture plate. The complete medium (DMEM and Ham’s F12, fetal bovine serum, antibiotic and essential amino acids) was added to the culture plates. The primary culture (PKW-LWH) cells were verified as fibroblast by vimentin and karyotype analyses, which revealed 42 autosomes and two sex chromosomes X and Y. Following transfection of PKW-LWH cells with a plasmid encoding, the SV40 large T-antigens and the transfected cells were isolated and expanded. Using RT-PCR, western blot, immunofluorescence analysis and SV40 large T-antigen stability was confirmed. The cell proliferation rate of the fibroblast cells, PKW-LWHT was faster than the primary cells PKW-LWH with the doubling time 68.9h and 14.4h, respectively. In this study, we established PKW dermal fibroblast cell line for the first time, providing a unique opportunity for in vitro studies on the effects of environmental pollutants and pathogens that could be determined in PKW and/or Cetaceans.
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影响因子：
5.9
作者：
Wang Y;Wu MH;Cheung MPL;Sham MH;Akiyama H;Chan D;Cheah KSE;Cheung M
通讯作者：
Cheung M
DOI：
10.1002/ar.a.20266
发表时间：
2005-12-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Yu, J;Kindy, MS;Gattoni-Celli, S
通讯作者：
Gattoni-Celli, S
影响因子：
3
作者：
Sakai, D;Mochida, J;Hotta, T
通讯作者：
Hotta, T
影响因子：
6.3
作者：
Sutherland, W. J.;Adams, W. M.;Watkinson, A. R.
通讯作者：
Watkinson, A. R.
印度太平洋座头海豚最大种群的人口统计和种群趋势
影响因子：
5.9
作者：
Huang, Shiang-Lin;Karczmarski, Leszek;Wu, Yuping
通讯作者：
Wu, Yuping