Mapping Loci That Control Tuber and Foliar Symptoms Caused by PVY in Autotetraploid Potato (Solanum tuberosum L.).
Mapping Loci That Control Tuber and Foliar Symptoms Caused by PVY in Autotetraploid Potato (Solanum tuberosum L.).
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DOI：
10.1534/g3.117.300264
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发表时间：
2017-11-06
期刊：
影响因子：
--
通讯作者：
Gray S
中科院分区：
文献类型：
--
作者：
da Silva WL;Ingram J;Hackett CA;Coombs JJ;Douches D;Bryan GJ;De Jong W;Gray S
马铃薯块茎坏死环斑病（Potato tuber necrotic ringspot disease，PTNRD）是由马铃薯Y病毒（Potato virus Y，PVYNTN）块茎坏死株系引起的马铃薯块茎畸形。PTNRD对块茎质量和适销性产生负面影响，并对全世界的种子和商业马铃薯生产构成严重威胁。品种Waneta和Pike的PVYNTN症状不同：Waneta表现出严重的PTNRD和叶片花叶与静脉和叶片坏死，而Pike不表达PTNRD和花叶是唯一的叶片症状。为了绘制影响块茎和叶片症状的基因座，将Waneta和Pike之间杂交的236个F1后代接种PVYNTN分离物NY090029，并使用12，808个马铃薯SNP进行基因分型。监测叶片症状类型和严重程度10周，同时在收获时和储存60天后再次评估块茎的PTNRD表达。成对相关分析表明PTNRD与静脉坏死之间存在强相关性（τ = 0.4195）。QTL分析显示主效QTL位于第4和第5染色体上的花叶，4 PTNRD，和5叶坏死症状。定位与PVY相关症状相关的QTL为育种者开发可用于消除具有不期望表型的马铃薯克隆的标记提供了基础，那些可能发展PTNRD或PVY的无菌携带者。
Potato tuber necrotic ringspot disease (PTNRD) is a tuber deformity associated with infection by the tuber necrotic strain of Potato virus Y (PVYNTN). PTNRD negatively impacts tuber quality and marketability, and poses a serious threat to seed and commercial potato production worldwide. PVYNTN symptoms differ in the cultivars Waneta and Pike: Waneta expresses severe PTNRD and foliar mosaic with vein and leaf necrosis, whereas Pike does not express PTNRD and mosaic is the only foliar symptom. To map loci that influence tuber and foliar symptoms, 236 F1 progeny of a cross between Waneta and Pike were inoculated with PVYNTN isolate NY090029 and genotyped using 12,808 potato SNPs. Foliar symptom type and severity were monitored for 10 wk, while tubers were evaluated for PTNRD expression at harvest and again after 60 d in storage. Pairwise correlation analyses indicate a strong association between PTNRD and vein necrosis (τ = 0.4195). QTL analyses revealed major-effect QTL on chromosomes 4 and 5 for mosaic, 4 for PTNRD, and 5 for foliar necrosis symptoms. Locating QTL associated with PVY-related symptoms provides a foundation for breeders to develop markers that can be used to eliminate potato clones with undesirable phenotypes, e.g., those likely to develop PTNRD or to be symptomless carriers of PVY.
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影响因子：
5.4
作者：
Hackett, Christine A.;Bradshaw, John E.;Bryan, Glenn J.
通讯作者：
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影响因子：
4.5
作者：
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作者：
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DOI：
10.1534/g3.114.012245
发表时间：
2014-08-21
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
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通讯作者：
Brummer EC
影响因子：
5.4
作者：
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通讯作者：
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