Xpf suppresses the mutagenic consequences of phagocytosis in Dictyostelium.
Xpf suppresses the mutagenic consequences of phagocytosis in Dictyostelium.
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DOI：
10.1242/jcs.196337
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发表时间：
2016-12-15
影响因子：
4
通讯作者：
Patel KJ
中科院分区：
文献类型：
--
作者：
Pontel LB;Langenick J;Rosado IV;Zhang XY;Traynor D;Kay RR;Patel KJ
随着时间的推移，突变在所有生物体的基因组中积累。这些变化促进了遗传多样性，但也加速了衰老和癌症的发生。食物是诱变剂的常见来源，但对营养因素如何引起食用生物体的持久遗传变化知之甚少。在这里，我们描述了一个不寻常的基因之间的相互作用，DNA修复的单细胞阿米巴Dictyosteelium discoideum和它的天然细菌食物来源。我们发现，缺乏DNA修复核酸酶Xpf（xpf−）的网骨藻在以细菌为食时表现出严重和特异性的生长缺陷。尽管精通细菌的吞噬和消化，但随着时间的推移，以细菌为食的xpf−网骨藻停止生长，在许多情况下死亡。Xpf核酸酶活性是使用细菌食物源持续生长所必需的。此外，这种食物来源的摄入导致xpf−网骨藻基因组中突变的惊人积累。因此，这项工作将盘基网柄菌建立为剖析营养遗传毒性的模型遗传系统，从而深入了解吞噬作用如何诱导突变并损害生存适应性。 DNA修复核酸酶Xpf有助于维持细菌吞噬过程中的基因组的完整性，在变形虫Dictyosteoba。
As time passes, mutations accumulate in the genomes of all living organisms. These changes promote genetic diversity, but also precipitate ageing and the initiation of cancer. Food is a common source of mutagens, but little is known about how nutritional factors cause lasting genetic changes in the consuming organism. Here, we describe an unusual genetic interaction between DNA repair in the unicellular amoeba Dictyostelium discoideum and its natural bacterial food source. We found that Dictyostelium deficient in the DNA repair nuclease Xpf (xpf−) display a severe and specific growth defect when feeding on bacteria. Despite being proficient in the phagocytosis and digestion of bacteria, over time, xpf− Dictyostelium feeding on bacteria cease to grow and in many instances die. The Xpf nuclease activity is required for sustained growth using a bacterial food source. Furthermore, the ingestion of this food source leads to a striking accumulation of mutations in the genome of xpf− Dictyostelium. This work therefore establishes Dictyostelium as a model genetic system to dissect nutritional genotoxicity, providing insight into how phagocytosis can induce mutagenesis and compromise survival fitness. The DNA repair nuclease Xpf helps to maintain the integrity of the genome during bacterial phagocytosis in the amoeba Dictyostelium.
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影响因子：
7.7
作者：
Bloomfield G;Traynor D;Sander SP;Veltman DM;Pachebat JA;Kay RR
通讯作者：
Kay RR
影响因子：
4.5
作者：
Zhang XY;Langenick J;Traynor D;Babu MM;Kay RR;Patel KJ
通讯作者：
Patel KJ
影响因子：
64.8
作者：
Rossi, Derrick J.;Bryder, David;Weissman, Irving L.
通讯作者：
Weissman, Irving L.
影响因子：
14.8
作者：
Fey, Petra;Kowal, Anthony S.;Chisholm, Rex L.
通讯作者：
Chisholm, Rex L.
DOI：
10.1016/0165-1161(80)90176-4
发表时间：
1980-01-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
PODGORSKI, G;DEERING, RA
通讯作者：
DEERING, RA