A transcriptional analysis of carotenoid, chlorophyll and plastidial isoprenoid biosynthesis genes during development and osmotic stress responses in Arabidopsis thaliana.
A transcriptional analysis of carotenoid, chlorophyll and plastidial isoprenoid biosynthesis genes during development and osmotic stress responses in Arabidopsis thaliana.
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DOI：
10.1186/1752-0509-5-77
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发表时间：
2011-05-19
影响因子：
--
通讯作者：
Wurtzel ET
中科院分区：
文献类型：
--
作者：
Meier S;Tzfadia O;Vallabhaneni R;Gehring C;Wurtzel ET
类胡萝卜素是纯的类异戊二烯，是光合机构的基本组成部分，在叶绿体中与叶绿素协同合成。然而，很少有人知道的机制，调节类胡萝卜素的生物合成或协调这种合成与叶绿素和其他质体合成类异戊二烯衍生的化合物，包括醌，赤霉酸和脱落酸的机制。在这里，一个全面的个人类胡萝卜素和类异戊二烯相关的生物合成途径基因的转录分析进行，以阐明这些化合物的协调合成中的转录调控的作用，并确定调控成分，可能介导这一过程中拟南芥。全球微阵列表达相关性分析表明，八氢番茄红素合成酶基因，编码的第一个专用和限速酶的胡萝卜素，是高度共表达与许多光合相关的基因，包括许多类异戊二烯相关的生物合成途径基因。化学和突变体分析表明，诱导萌发后的共表达基因依赖于赤霉素和油菜素内酯（BR），但抑制脱落酸（阿坝）。突变体分析进一步揭示，在黑暗生长的植物中，许多基因的表达被光敏色素相互作用转录因子（PIF）抑制，并被光敏色素激活，光敏色素反过来降解PIF并介导基因的协调诱导。发现PSY和共表达基因的启动子含有富集的推定BR-生长素应答元件和G盒，其结合PIF，进一步支持BR和PIF在调节基因表达中的作用。在干旱胁迫的根组织中，卡尔文循环、甲基β 4-磷酸途径和类胡萝卜素生物合成基因的转录被诱导，并以与阿坝生物合成的类胡萝卜素前体合成增加一致的方式与叶绿素生物合成基因的转录解偶联。在所有检查的组织中，β-胡萝卜素羟化酶转录水平的诱导与阿坝需求的增加有关。该分析提供了令人信服的证据表明，类异戊二烯相关的生物合成途径基因的协调转录调控在协调功能相关的叶绿体定位的类异戊二烯衍生化合物的合成中起着重要作用。
The carotenoids are pure isoprenoids that are essential components of the photosynthetic apparatus and are coordinately synthesized with chlorophylls in chloroplasts. However, little is known about the mechanisms that regulate carotenoid biosynthesis or the mechanisms that coordinate this synthesis with that of chlorophylls and other plastidial synthesized isoprenoid-derived compounds, including quinones, gibberellic acid and abscisic acid. Here, a comprehensive transcriptional analysis of individual carotenoid and isoprenoid-related biosynthesis pathway genes was performed in order to elucidate the role of transcriptional regulation in the coordinated synthesis of these compounds and to identify regulatory components that may mediate this process in Arabidopsis thaliana. A global microarray expression correlation analysis revealed that the phytoene synthase gene, which encodes the first dedicated and rate-limiting enzyme of carotenogenesis, is highly co-expressed with many photosynthesis-related genes including many isoprenoid-related biosynthesis pathway genes. Chemical and mutant analysis revealed that induction of the co-expressed genes following germination was dependent on gibberellic acid and brassinosteroids (BR) but was inhibited by abscisic acid (ABA). Mutant analyses further revealed that expression of many of the genes is suppressed in dark grown plants by Phytochrome Interacting transcription Factors (PIFs) and activated by photoactivated phytochromes, which in turn degrade PIFs and mediate a coordinated induction of the genes. The promoters of PSY and the co-expressed genes were found to contain an enrichment in putative BR-auxin response elements and G-boxes, which bind PIFs, further supporting a role for BRs and PIFs in regulating expression of the genes. In osmotically stressed root tissue, transcription of Calvin cycle, methylerythritol 4-phosphate pathway and carotenoid biosynthesis genes is induced and uncoupled from that of chlorophyll biosynthesis genes in a manner that is consistent with the increased synthesis of carotenoid precursors for ABA biosynthesis. In all tissues examined, induction of β-carotene hydroxylase transcript levels are linked to an increased demand for ABA. This analysis provides compelling evidence to suggest that coordinated transcriptional regulation of isoprenoid-related biosynthesis pathway genes plays a major role in coordinating the synthesis of functionally related chloroplast localized isoprenoid-derived compounds.
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影响因子：
4.1
作者：
Hirooka, K;Bamba, T;Kobayashi, A
通讯作者：
Kobayashi, A
影响因子：
14.9
作者：
Al-Shahrour F;Minguez P;Tárraga J;Medina I;Alloza E;Montaner D;Dopazo J
通讯作者：
Dopazo J
影响因子：
5.8
作者：
Al-Shahrour, F;Díaz-Uriarte, R;Dopazo, J
通讯作者：
Dopazo, J
影响因子：
7.4
作者：
Goda, H;Shimada, Y;Yoshida, S
通讯作者：
Yoshida, S
影响因子：
3.9
作者：
Ghassemian, Majid;Lutes, Jason;Lange, B. Markus
通讯作者：
Lange, B. Markus