A comparison of two probiotic strains of bifidobacteria in premature infants.
A comparison of two probiotic strains of bifidobacteria in premature infants.
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DOI：
10.1016/j.jpeds.2013.07.017
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发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Mills DA
中科院分区：
文献类型：
--
作者：
Underwood MA;Kalanetra KM;Bokulich NA;Lewis ZT;Mirmiran M;Tancredi DJ;Mills DA
目的：确定两种益生菌双歧杆菌对喂养母乳或配方奶的早产儿粪便微生物区系的影响。在两个第一阶段临床试验中，12名接受配方奶喂养的早产儿被随机分配到婴儿双歧杆菌或动物双歧杆菌乳汁中，在五周的时间里增加剂量。在第二项试验中，9名接受母乳喂养的早产儿分别接受了两种双歧杆菌，每种双歧杆菌之间有一周的洗涤期。对每个婴儿的连续粪便标本进行末端限制性片段长度多态性和定量聚合酶链式反应的细菌组成分析。在配方奶粉喂养的婴儿中，接受婴儿双歧杆菌的婴儿比接受乳杆菌的婴儿的粪便双歧杆菌增加得更多。这种差异在每天两次1.4×109 CFU剂量时最为显著(p<；0.05)。在接受婴儿芽孢杆菌治疗的婴儿中，细菌多样性随剂量/时间的增加而改善。在母乳喂养的婴儿中，服用双歧杆菌后粪便双歧杆菌的增加和γ-变形杆菌的减少比给予乳杆菌的增加更大。无论使用何种益生菌，在母乳喂养的婴儿中，长双歧杆菌(包括婴儿双歧杆菌，但不包括乳双歧杆菌)是主要的双歧杆菌。在配方奶喂养的早产儿和母乳喂养的早产儿中，婴儿杆菌在粪便微生物区系定植方面比乳酸杆菌更有效。母乳和婴儿双歧杆菌的组合产生了最高水平的粪便双歧杆菌。
To determine the impact of two probiotic bifidobacteria on the fecal microbiota of premature infants fed either human milk or formula. In the first of two phase 1 clinical trials, twelve premature infants receiving formula feedings were randomly assigned to receive either Bifidobacterium longum ssp infantis or Bifidobacterium animalis ssp lactis in increasing doses over a five week period. In the second, nine premature infants receiving their mother’s milk received each of the two bifidobacteria for two weeks separated by a one week wash out period. Serial stool specimens from each infant were analyzed by terminal restriction fragment length polymorphism and quantitative polymerase chain reaction for bacterial composition. Among the formula-fed infants, there was a greater increase in fecal bifidobacteria among infants receiving B. infantis than those receiving B. lactis. This difference was most marked at a dose of 1.4 × 109 cfu twice daily (p < 0.05). Bacterial diversity improved over dose/time in those infants receiving B. infantis. Among the human milk-fed infants, greater increases in fecal bifidobacteria and decreases in γ-Proteobacteria followed administration of B. infantis than B. lactis. The B. longum group (which includes B. infantis but not B. lactis) was the dominant bifidobacteria among the human milk-fed infants, regardless of the probiotic administered. B. infantis was more effective at colonizing the fecal microbiota than B. lactis in both formula-fed and human milk-fed premature infants. The combination of human milk plus B. infantis resulted in the highest fecal levels of bifidobacteria.
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DOI：
10.1097/mpg.0b013e3182105850
发表时间：
2011-06-01
影响因子：
2.9
作者：
Hascoet, Jean-Michel;Hubert, Claire;Steenhout, Philippe G.
通讯作者：
Steenhout, Philippe G.
影响因子：
5.1
作者：
Kuppala, Venkata S.;Meinzen-Derr, Jareen;Morrow, Ardythe L.;Schibler, Kurt R.
通讯作者：
Schibler, Kurt R.
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
5.1
作者：
Abdo, Z;Schüette, UME;Joyce, P
通讯作者：
Joyce, P
影响因子：
4.7
作者：
Larsen, C. N.;Nielsen, S.;Michaelsen, K. F.
通讯作者：
Michaelsen, K. F.