Wolbachia strain wPip yields a pattern of cytoplasmic incompatibility enhancing a Wolbachia-based suppression strategy against the disease vector Aedes albopictus.
Wolbachia strain wPip yields a pattern of cytoplasmic incompatibility enhancing a Wolbachia-based suppression strategy against the disease vector Aedes albopictus.
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Wolbachia菌株WPIP产生了一种细胞质不相容性的模式，从而增强了针对疾病媒介艾德斯白化病的基于Wolbachia的抑制策略。
DOI：
10.1186/1756-3305-5-254
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发表时间：
2012-11-12
影响因子：
3.2
通讯作者：
Dobson SL
中科院分区：
文献类型：
--
作者：
Calvitti M;Moretti R;Skidmore AR;Dobson SL
细胞质不亲和性（CI）在自然界中由沃尔巴克氏体细菌诱导，导致条件性雄性不育。先前的研究表明，自然共感染疾病媒介蚊子白纹伊蚊（亚洲虎蚊）的两种沃尔巴克氏体菌株（wAlbA和wAlbB）可以被来自库蚊的wPip沃尔巴克氏体菌株所取代。由于沃尔巴克氏体为基础的矢量控制策略依赖于CI的强度和一致性，需要更深入地了解CI之间的关系wPip，wAlbA和wAlbB沃尔巴克氏体在Ae。白纹伊蚊本工作包括在意大利和美国进行的一系列协作杂交，以研究“wPip”感染的Ae之间的CI关系。白纹伊蚊株（ARwP）和重叠感染的SR株。的Ae。在意大利试验中使用的白纹伊蚊菌株是wPip感染的ARwP菌株（ARwPIT）、重复感染的SR菌株和非共生AR菌株。为了了解观察到的CI模式，在美国进行的杂交实验集中于研究ARwP（ARwPUS）和人工产生的单一感染株系之间的CI关系，在特定的HTA和HTB中，分别仅携带wAlbA和wAlbB Wolbachia。本文报道了在ARwP与SR系杂交试验中观察到的一种不寻常的CI模式。具体而言，ARwP雄性能够在野生型雌性的一生中的大部分时间内诱导完全不育，而SR雄性和ARwP雌性之间的杂交随着雄性衰老而变得部分可育。我们证明，观察到的CI感染率与SR男性年龄的减少，与先前描述的沃尔巴克氏体密度的减少，特别是wAlbA菌株，发生在老年重叠感染男性。本文报道的结果支持使用ARwP Ae。白纹伊蚊品系作为“现成不育雄性”的来源，作为γ辐射绝育雄性的替代品，用于针对亚洲虎蚊的自动杀灭策略。此外，在SR雄性和ARwP雌性之间的杂交中观察到的年龄依赖性CI减弱简化了下游努力以保持实验室ARwP菌落内的遗传变异性，迄今为止基于野生捕获的双重感染蚊子的抗生素处理，也降低了成本。
Cytoplasmic incompatibility (CI) is induced in nature by Wolbachia bacteria, resulting in conditional male sterility. Previous research demonstrated that the two Wolbachia strains (wAlbA and wAlbB) that naturally co-infect the disease vector mosquito Aedes albopictus (Asian tiger mosquito) can be replaced with the wPip Wolbachia strain from Culex pipiens. Since Wolbachia-based vector control strategies depend upon the strength and consistency of CI, a greater understanding is needed on the CI relationships between wPip, wAlbA and wAlbB Wolbachia in Ae. albopictus. This work consisted of a collaborative series of crosses carried out in Italy and in US to study the CI relationships between the “wPip” infected Ae. albopictus strain (ARwP) and the superinfected SR strain. The Ae. albopictus strains used in Italian tests are the wPip infected ARwP strain (ARwPIT), the superinfected SR strain and the aposymbiotic AR strain. To understand the observed pattern of CI, crossing experiments carried out in USA focused on the study of the CI relationships between ARwP (ARwPUS) and artificially-generated single infected lines, in specific HTA and HTB, harbouring only wAlbA and wAlbB Wolbachia respectively. The paper reports an unusual pattern of CI observed in crossing experiments between ARwP and SR lines. Specifically, ARwP males are able to induce full sterility in wild type females throughout most of their lifetime, while crosses between SR males and ARwP females become partially fertile with male aging. We demonstrated that the observed decrease in CI penetrance with SR male age, is related to the previously described decrease in Wolbachia density, in particular of the wAlbA strain, occurring in aged superinfected males. The results here reported support the use of the ARwP Ae. albopictus line as source of “ready-made sterile males”, as an alternative to gamma radiation sterilized males, for autocidal suppression strategies against the Asian tiger mosquito. In addition, the age dependent CI weakening observed in the crosses between SR males and ARwP females simplifies the downstream efforts to preserve the genetic variability within the laboratory ARwP colonies, to date based on the antibiotic treatment of wild captured superinfected mosquitoes, also reducing the costs.
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影响因子：
2.1
作者：
Bellini, Romeo;Albieri, Alessandro;Maini, Stefano
通讯作者：
Maini, Stefano
影响因子：
3.8
作者：
Duron, O.;Fort, P.;Weill, M.
通讯作者：
Weill, M.
影响因子：
2.1
作者：
Calvitti, Maurizio;Moretti, Riccardo;Dobson, Stephen L.
通讯作者：
Dobson, Stephen L.
影响因子：
3.2
作者：
Brelsfoard CL;Dobson SL
通讯作者：
Dobson SL
影响因子：
2.1
作者：
Benedict, Mark Q.;Levine, Rebecca S.;Lounibos, L. Philip
通讯作者：
Lounibos, L. Philip