Placental expression of glucose transporters GLUT-1, GLUT-3, GLUT-8 and GLUT-12 in pregnancies complicated by gestational and type 1 diabetes mellitus.
Placental expression of glucose transporters GLUT-1, GLUT-3, GLUT-8 and GLUT-12 in pregnancies complicated by gestational and type 1 diabetes mellitus.
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DOI：
10.1111/jdi.13680
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发表时间：
2022-03
影响因子：
3.2
通讯作者：
Wielgoś M
中科院分区：
文献类型：
--
作者：
Stanirowski PJ;Szukiewicz D;Majewska A;Wątroba M;Pyzlak M;Bomba-Opoń D;Wielgoś M
本研究的目的是评估合并良好控制妊娠期（GDM）和1型妊娠期糖尿病（PGDM）的足月妊娠患者胎盘中葡萄糖转运体GLUT‐1、GLUT‐3、GLUT‐8和GLUT‐12的表达。共从诊断为GDM的患者（n = 60）、PGDM患者（n = 20）和非糖尿病对照组（n = 23）中获得103份胎盘样本。对染色的胎盘切片进行计算机辅助定量形态测定，以确定所选GLUT蛋白的表达。用于鉴定GLUT‐1、GLUT‐3、GLUT‐8和GLUT‐12的免疫组织化学技术揭示了胎盘组织中所有葡萄糖转运蛋白的存在。对血管密度匹配的胎盘样本进行的形态计量学评估显示，与GDM和对照组相比，PGDM患者中GLUT - 1蛋白的表达显著增加（P < 0.05）。至于其他GLUT亚型的表达，在糖尿病患者和对照组之间没有观察到统计学上的显著差异。胎儿出生体重与PGDM组GLUT‐1蛋白表达呈正相关（rho = 0.463, P < 0.05），对照组GLUT‐12蛋白表达呈正相关（rho = 0.481, P < 0.05）。在足月妊娠合并控制良好的GDM/PGDM时，胎盘中转运蛋白GLUT‐3、GLUT‐8和GLUT‐12的表达不受影响。在1型PGDM女性中，GLUT‐1的表达增加可能导致该人群中较大胎儿的发生率升高。在妊娠合并妊娠期/妊娠期糖尿病时，胎盘GLUT表达的改变可能导致进入胎儿循环的葡萄糖通量增加，从而导致胎儿巨大儿。研究结果表明，与妊娠期糖尿病患者和健康对照组相比，妊娠期糖尿病患者的GLUT - 1表达明显增加。此外，在前一组患者中，GLUT‐1的表达与胎儿出生体重呈正相关。关于GLUT‐3、8和12蛋白，糖尿病和正常血糖孕妇之间没有观察到显著差异。1型妊娠期糖尿病妇女中GLUT - 1表达升高可能导致该人群中较大胎儿的发生率升高。
The aim of the present study was to evaluate the placental expression of glucose transporters GLUT‐1, GLUT‐3, GLUT‐8 and GLUT‐12 in term pregnancies complicated by well‐controlled gestational (GDM) and type 1 pregestational diabetes mellitus (PGDM). A total of 103 placental samples were obtained from patients diagnosed with GDM (n = 60), PGDM (n = 20) and a non‐diabetic control group (n = 23). Computer‐assisted quantitative morphometry of stained placental sections was performed to determine the expression of selected GLUT proteins. Immunohistochemical techniques used for the identification of GLUT‐1, GLUT‐3, GLUT‐8 and GLUT‐12 revealed the presence of all glucose transporters in the placental tissue. Morphometric evaluation performed for the vascular density‐matched placental samples demonstrated a significant increase in the expression of GLUT‐1 protein in patients with PGDM as compared to GDM and control groups (P < 0.05). With regard to the expression of the other GLUT isoforms, no statistically significant differences were observed between patients from the diabetic and control populations. Positive correlations between fetal birthweight and the expression of GLUT‐1 protein in the PGDM group (rho = 0.463, P < 0.05) and GLUT‐12 in the control group (rho = 0.481, P < 0.05) were noted. In term pregnancies complicated by well‐controlled GDM/PGDM, expression of transporters GLUT‐3, GLUT‐8 and GLUT‐12 in the placenta remains unaffected. Increased expression of GLUT‐1 among women with type 1 PGDM might contribute to a higher rate of macrosomic fetuses in this population. In pregnancies complicated by gestational/pregestational diabetes mellitus, alterations in the placental GLUT expression might lead to an increased glucose flux into the fetal circulation, and thus fetal macrosomia. Study results demonstrated significantly increased GLUT‐1 expression in patients with pregestational diabetes mellitus as compared to women affected by GDM and healthy controls. In addition, in the former group of patients, GLUT‐1 expression was positively correlated with the fetal birthweight. With regard to GLUT‐3, 8 and 12 proteins, no significant differences were observed between diabetic and normoglycemic pregnancies. Increased expression of GLUT‐1 among women with type 1 pregestational diabetes mellitus might contribute to a higher rate of macrosomic fetuses in this population.
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影响因子：
3.8
作者：
Huynh, J.;Dawson, D.;Roberts, D.;Bentley-Lewis, R.
通讯作者：
Bentley-Lewis, R.
影响因子：
5.1
作者：
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作者：
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Devaskar, S. U.
影响因子：
2.1
作者：
Kainulainen, H;Jarvinen, T;Heinonen, PK
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影响因子：
4.1
作者：
Castillo-Castrejon M;Yamaguchi K;Rodel RL;Erickson K;Kramer A;Hirsch NM;Rolloff K;Jansson T;Barbour LA;Powell TL
通讯作者：
Powell TL