Increased intratumoral neutrophil in colorectal carcinomas correlates closely with malignant phenotype and predicts patients' adverse prognosis.
Increased intratumoral neutrophil in colorectal carcinomas correlates closely with malignant phenotype and predicts patients' adverse prognosis.
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结直肠癌瘤内中性粒细胞增多与恶性表型密切相关，可预测患者的不良预后。
DOI：
10.1371/journal.pone.0030806
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Xie D
中科院分区：
文献类型：
--
作者：
Rao HL;Chen JW;Li M;Xiao YB;Fu J;Zeng YX;Cai MY;Xie D
大量证据表明，炎性细胞的存在在人类肿瘤的发展和/或进展中起关键作用。中性粒细胞是肿瘤中常见的炎性细胞，然而，对于结直肠癌（CRC）中肿瘤内中性粒细胞的浸润及其对患者预后的影响知之甚少。本研究应用组织芯片和免疫组化方法研究结直肠癌组织中CD 66 b+中性粒细胞的表达与结直肠癌预后的关系。根据受试者工作特征曲线分析，当肿瘤内CD 66 b+中性粒细胞平均计数大于60个/TMA斑点时，CRC中高肿瘤内CD 66 b+中性粒细胞的临界值被定义。在我们的研究中，在104/229（45.4%）的CRC和29/229（12.7%）的邻近粘膜组织中观察到高肿瘤内CD 66 b+中性粒细胞。进一步相关分析显示，肿瘤内中性粒细胞增高与pT状态、pM状态及临床分期呈正相关（P<0.05）。在单因素生存分析中，发现肿瘤内高中性粒细胞与患者生存期缩短显著相关（P<0.0001）。在不同亚群的结直肠癌患者中，II期、III期、2级、3级、pT 1、pT 2、pN 0和pN 1患者的肿瘤内中性粒细胞也是预后指标（P<0.05）。重要的是，在多变量分析中，高瘤内中性粒细胞被评估为独立的预后因素（P<0.05）。我们的研究结果提供的证据表明，增加的肿瘤内中性粒细胞在结直肠癌的恶性表型的收购可能是重要的，表明肿瘤内中性粒细胞的存在是一个独立的因素与结直肠癌患者预后不良。
Substantial evidence suggests that the presence of inflammatory cells plays a critical role in the development and/or progression of human tumors. Neutrophils are the common inflammatory cells in tumors; however, the infiltration of intratumoral neutrophils in colorectal carcinoma (CRC) and its effect on CRC patients' prognosis are poorly understood. In this study, the methods of tissue microarray and immunohistochemistry (IHC) were used to investigate the prognostic significance of intratumoral CD66b+ neutrophil in CRC. According to receiver operating characteristic curve analysis, the cutoff score for high intratumoral CD66b+ neutrophil in CRC was defined when the mean counts were more than 60 per TMA spot. In our study, high intratumoral CD66b+ neutrophil was observed in 104/229 (45.4%) of CRCs and in 29/229 (12.7%) of adjacent mucosal tissues. Further correlation analysis showed that high intratumoral neutrophil was positively correlated with pT status, pM status and clinical stage (P<0.05). In univariate survival analysis, a significant association between high intratumoral neutrophil and shortened patients' survival was found (P<0.0001). In different subsets of CRC patients, intratumoral neutrophil was also a prognostic indicator in patients with stage II, stage III, grade 2, grade 3, pT1, pT2, pN0 and pN1 (P<0.05). Importantly, high intratumoral neutrophil was evaluated as an independent prognostic factor in multivariate analysis (P<0.05). Our results provide evidence that increased intratumoral neutrophil in CRC may be important in the acquisition of a malignant phenotype, indicating that the presence of intratumoral neutrophil is an independent factor for poor prognosis of patients with CRC.
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影响因子：
29.4
作者：
GRISHAM, MB;WARE, K;YAMADA, T
通讯作者：
YAMADA, T
影响因子：
4.6
作者：
Eck, M;Schmausser, B;Müller-Hermelink, HK
通讯作者：
Müller-Hermelink, HK
影响因子：
45.3
作者：
Jensen, Hanne Krogh;Donskov, Frede;von der Maase, Hans
通讯作者：
von der Maase, Hans
PinX1蛋白表达降低与肿瘤发生发展相关，是卵巢癌新的独立不良预后因素
影响因子：
5.7
作者：
Cai, Mu-Yan;Zhang, Bin;Xie, Dan
通讯作者：
Xie, Dan
DOI：
10.1158/1078-0432.ccr-11-0226
发表时间：
2011-07-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Sun Y;Yokoi K;Li H;Gao J;Hu L;Liu B;Chen K;Hamilton SR;Fan D;Sun B;Zhang W
通讯作者：
Zhang W