SARS-CoV-2 RNA in exhaled air of hospitalized COVID-19 patients.

SARS-CoV-2 RNA in exhaled air of hospitalized COVID-19 patients.
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DOI:
10.1038/s41598-022-13008-4
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发表时间:
2022-05-30
期刊:
影响因子:
4.6
通讯作者:
--
中科院分区:
综合性期刊3区
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--
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了解传染性是准确管理住院COVID-19患者的关键。流行病学研究表明,除了通过飞沫传播外,产气SARS-CoV-2传播也有助于感染的传播。然而,呼出的空气中存在病毒尚未得到充分证明。在大流行的情况下,需要低技术含量的一次性和用户友好的床边设备,而市售的采样器不适合应用于呼吸窘迫患者。我们纳入了49名住院的COVID-19患者,并使用一次性模块化呼吸采样器测量呼出空气样本中的SARS-CoV-2 RNA载量,并将其与鼻咽咽拭子和唾液的SARS-CoV-2 RNA载量进行比较。使用模块化呼吸采样器进行呼出空气采样已被证明在临床COVID-19环境中是可行的,并在25%的患者中证明了病毒检测。
Knowledge about contagiousness is key to accurate management of hospitalized COVID-19 patients. Epidemiological studies suggest that in addition to transmission through droplets, aerogenic SARS-CoV-2 transmission contributes to the spread of infection. However, the presence of virus in exhaled air has not yet been sufficiently demonstrated. In pandemic situations low tech disposable and user-friendly bedside devices are required, while commercially available samplers are unsuitable for application in patients with respiratory distress. We included 49 hospitalized COVID-19 patients and used a disposable modular breath sampler to measure SARS-CoV-2 RNA load in exhaled air samples and compared these to SARS-CoV-2 RNA load of combined nasopharyngeal throat swabs and saliva. Exhaled air sampling using the modular breath sampler has proven feasible in a clinical COVID-19 setting and demonstrated viral detection in 25% of the patients.
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