SARS-CoV-2 RT-PCR positivity of individuals subsequent to completing quarantine upon entry into a country during a transmission-free period.
SARS-CoV-2 RT-PCR positivity of individuals subsequent to completing quarantine upon entry into a country during a transmission-free period.
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DOI：
10.1016/j.tmaid.2022.102271
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发表时间：
2022-03
影响因子：
12
通讯作者：
Phuphuakrat A
中科院分区：
文献类型：
--
作者：
Srichatrapimuk S;Chookajorn T;Kochakarn T;Kirdlarp S;Pasomsub E;Chantratita W;Iamsirithaworn S;Kunakorn M;Thitithanyanont A;Sungkanuparph S;Phuphuakrat A
在当前的2019冠状病毒病（COVID-19）大流行期间，许多国家要求旅行者在跨境旅行前接受严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）的逆转录聚合酶链反应（RT-PCR）检测。然而，在从COVID-19中康复的人中，RT-PCR阳性可以持续很长一段时间。我们描述了三个病例，他们在没有当地传播的时期内在泰国寻求适合飞行的证明，但对SARS-CoV-2的RT-PCR检测呈阳性。所有人都是从一个COVID-19疫情活跃的国家返回的。他们的临床过程进行了描述;阳性鼻咽拭子样本进行病毒分离和全基因组测序（WGS）处理;血清学以及中和抗体进行了评估。进行了接触者追踪，以确定这三个病例的密切接触者之间的本土传播证据。这三个病例都完全没有症状。胸部计算机断层扫描与COVID-19肺炎不相容;细胞培养未能拯救有复制能力的病毒; WGS显示鼻咽拭子样本中的病毒遗传物质片段;血清学检测显示抗体水平稳定，同时存在中和抗体，表明既往感染，传播风险可忽略不计。接触者追踪未发现高风险密切接触者的传播。RT-PCR检测SARS-CoV-2阳性可检测到病毒基因片段。在这种情况下发放旅行证是有问题的。血清学测试可以帮助确定过去的感染。有必要为签发COVID-19安全旅行证书制定一套切实可行的可接受准则。
During the current coronavirus disease 2019 (COVID-19) pandemic, many countries require travellers to undergo a reverse transcription-polymerase chain reaction (RT-PCR) testing for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) before travelling across borders. However, in persons having recovered from COVID-19, RT-PCR positivity can persist for an extended period. We describe three cases who sought fit-to-fly certificates in Thailand during the period free of local transmission but were tested positive for RT-PCR for SARS-CoV-2. All had returned from a country with an active outbreak of COVID-19. Their clinical courses are described; positive nasopharyngeal swab samples were processed for viral isolation and whole-genome sequencing (WGS); and serology as well as neutralizing antibody were assessed. The contact tracing was carried out for determining evidence of indigenous transmission among close contacts of those three cases. All three cases were completely asymptomatic. Chest computerized tomography was not compatible with COVID-19 pneumonia; cell cultures failed to rescue replication-competent virus; WGS revealed fragmented viral genetic material from nasopharyngeal swab samples; and serological tests demonstrated stable levels of antibodies, together with the presence of neutralizing antibody, suggesting past infection with negligible transmission risk. Contact tracing identified no transmission in high-risk close contact individuals. RT-PCR positivity for SARS-CoV-2 might detect fragmented viral genome. Issuance of a travel certificate in these circumstances is problematic. Serology tests can help to define past infection. A practical acceptable set of guidelines for issuance of a COVID-19 safety travel certification is a necessity.
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影响因子：
82.9
作者：
Long, Quan-Xin;Liu, Bai-Zhong;Huang, Ai-Long
通讯作者：
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