TopA, the Sulfolobus solfataricus topoisomerase III, is a decatenase.
TopA, the Sulfolobus solfataricus topoisomerase III, is a decatenase.
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TOPA，磺胺solfataricus topoisomerase III，是一种deTANEPASE。
DOI：
10.1093/nar/gkx1247
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发表时间：
2018-01-25
影响因子：
14.9
通讯作者：
Nadal M
中科院分区：
文献类型：
--
作者：
Bizard AH;Yang X;Débat H;Fogg JM;Zechiedrich L;Strick TR;Garnier F;Nadal M
DNA拓扑异构酶是参与所有DNA过程的必需酶，其中IA型拓扑异构酶是维持基因组稳定性的关键因素。超嗜热古菌Sulfolobus solfataricus含有三种拓扑异构酶IA，其中一种经典的拓扑异构酶称为TopA。SsoTopA在解链DNA链烷方面非常有效，将SsoTopA分组到拓扑异构酶III家族中。SsoTopA在很宽的温度范围内都有活性，在高达85°C的温度下，它会产生高度解绕的DNA。在更高的温度下，SsoTopA将两条DNA链分开。因此，根据温度，SsoTopA能够防止或促进DNA解链。虽然典型的拓扑异构酶III需要一个单链DNA区域或缺口的一个圆圈decatenate他们，我们第一次表明，I型拓扑异构酶，SsoTopA，能够有效地解除共价封闭的catenanes，没有额外的合作伙伴。通过使用单分子实验，我们证明了SsoTopA需要存在一个短的单链DNA区域是有效的。SsoTopA的意外解链特性可能来自其捕获该解链区域的高能力。这指出了TopA在S. sulfataricus作为Sulfolobus中的十链烯酶。
DNA topoisomerases are essential enzymes involved in all the DNA processes and among them, type IA topoisomerases emerged as a key actor in the maintenance of genome stability. The hyperthermophilic archaeon, Sulfolobus solfataricus, contains three topoisomerases IA including one classical named TopA. SsoTopA is very efficient at unlinking DNA catenanes, grouping SsoTopA into the topoisomerase III family. SsoTopA is active over a wide range of temperatures and at temperatures of up to 85°C it produces highly unwound DNA. At higher temperatures, SsoTopA unlinks the two DNA strands. Thus depending on the temperature, SsoTopA is able to either prevent or favor DNA melting. While canonical topoisomerases III require a single-stranded DNA region or a nick in one of the circles to decatenate them, we show for the first time that a type I topoisomerase, SsoTopA, is able to efficiently unlink covalently closed catenanes, with no additional partners. By using single molecule experiments we demonstrate that SsoTopA requires the presence of a short single-stranded DNA region to be efficient. The unexpected decatenation property of SsoTopA probably comes from its high ability to capture this unwound region. This points out a possible role of TopA in S. solfataricus as a decatenase in Sulfolobus.
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