Characterization of a new Leishmania major strain for use in a controlled human infection model.
Characterization of a new Leishmania major strain for use in a controlled human infection model.
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DOI：
10.1038/s41467-020-20569-3
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发表时间：
2021-01-11
影响因子：
16.6
通讯作者：
Kaye PM
中科院分区：
文献类型：
--
作者：
Ashwin H;Sadlova J;Vojtkova B;Becvar T;Lypaczewski P;Schwartz E;Greensted E;Van Bocxlaer K;Pasin M;Lipinski KS;Parkash V;Matlashewski G;Layton AM;Lacey CJ;Jaffe CL;Volf P;Kaye PM
利什曼病被广泛认为是一种疫苗可预防的疾病，但进入关键的3期研究所需的成本以及哪些候选疫苗应进入人体研究的不确定性大大限制了这种被忽视的热带疾病疫苗开发的进展。受控人类感染模型（CHIM）为加速疫苗开发和更全面地了解疾病发病机制和保护相关因素提供了一条途径。在这里，我们描述了分离，表征和GMP生产的一个新的临床菌株利什曼原虫重大。两株新鲜L.来自以色列的主要品种最初通过基因组测序、小鼠体内感染性和药物敏感性以及白蛉的发育和传播能力进行比较，从而选择一种用于GMP生产。这项研究解决了利什曼病疫苗开发的主要障碍，为白蛉传播的皮肤利什曼病的CHIM研究提供了关键资源。受控人类感染模型（CHIM）为加速疫苗开发提供了一条途径。在这里，作者描述了临床利什曼原虫主要菌株的分离，表征和GMP生产，用作白蛉传播的皮肤利什曼病的CHIM研究的资源。
Leishmaniasis is widely regarded as a vaccine-preventable disease, but the costs required to reach pivotal Phase 3 studies and uncertainty about which candidate vaccines should be progressed into human studies significantly limits progress in vaccine development for this neglected tropical disease. Controlled human infection models (CHIMs) provide a pathway for accelerating vaccine development and to more fully understand disease pathogenesis and correlates of protection. Here, we describe the isolation, characterization and GMP manufacture of a new clinical strain of Leishmania major. Two fresh strains of L. major from Israel were initially compared by genome sequencing, in vivo infectivity and drug sensitivity in mice, and development and transmission competence in sand flies, allowing one to be selected for GMP production. This study addresses a major roadblock in the development of vaccines for leishmaniasis, providing a key resource for CHIM studies of sand fly transmitted cutaneous leishmaniasis. Controlled human infection models (CHIMs) provide a pathway for accelerating vaccine development. Here, the authors describe the isolation, characterization, and GMP manufacture of a clinical Leishmania major strain to be used as a resource for CHIM studies of sand fly transmitted cutaneous leishmaniasis.
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DOI：
10.1093/cid/ciy670
发表时间：
2019-04-08
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Gibani MM;Voysey M;Jin C;Jones C;Thomaides-Brears H;Jones E;Baker P;Morgan M;Simmons A;Gordon MA;Cerundolo V;Pitzer VE;Angus B;Levine MM;Darton TC;Pollard AJ
通讯作者：
Pollard AJ
DOI：
10.1080/00034983.1948.11685360
发表时间：
1948-01-01
影响因子：
--
作者：
ADLER, S;ZUCKERMAN, A
通讯作者：
ZUCKERMAN, A
影响因子：
0.5
作者：
Bufman, Hila;Sagi, Orli;Ben-Shimol, Shalom
通讯作者：
Ben-Shimol, Shalom
影响因子：
5.8
作者：
Coler, Rhea N.;Duthie, Malcolm S.;Reed, Steven G.
通讯作者：
Reed, Steven G.
影响因子：
82.9
作者：
Gibani, Malick M.;Jones, Elizabeth;Pollard, Andrew J.
通讯作者：
Pollard, Andrew J.